Abstract A glucose amperometric biosensor has been prepared by a multilayer deposition on glassy carbon electrode of Co3Mn-CO3 layered double hydroxide, oxidase (GOx) and biopolymer (carrageenan), respectively. The original transduction step consists in the electrocatalytic oxidation H2O2, enzymatically formed, inner-layer at 0.65 V/Ag-AgCl. sensitivity detection limit, obtained with this optim...

The European Diagnostic Working Group presented comprehensive diagnostic algorithms for cystic fibrosis (CF) and confirmed the fundamental role of the sweat test for the diagnosis of CF. However, several important differences between well-accepted guidelines for sweat testing 3 and the recommendations of the Working Group need to be discussed. An adequate sweat sampling volume depends on the sa...

Background: Transcription factors regulate gene expression by interacting with their specific DNA binding sites. Some transcription factors, particularly those involved in transcription initiation, always bind close to transcription start sites (TSS). Others have no such preference and are functional on sites even tens of thousands of base pairs (bp) away from the TSS. The Cyclic-AMP response e...

Nanoparticles have the advantage of a superior surface area to volume ratio, and thus such materials are useful for enzyme immobilization. A silver nanoparticle coated cotton fabric (AgNp-CF) is used immobilize camel liver catalase in present work. The effect loading levels AgNp inside fabrics on immobilization was investigated. results revealed that 6 mL level precursor (silver nitrate, 2 mM) ...

BACKGROUND & AIMS Intestinal inflammation is a hallmark of cystic fibrosis (CF). Administration of probiotics can reduce intestinal inflammation and the incidence of pulmonary exacerbations. We investigated the composition of intestinal microbiota in children with CF and analyzed its relationship with intestinal inflammation. We also investigated the microflora structure before and after Lactob...

BACKGROUND Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo. METHODS We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples t...

BACKGROUND Cystic fibrosis (CF) with pancreatic insufficiency is associated with poor absorption of fat and fat-soluble vitamins, including vitamin D. Pancreatic enzyme supplementation does not completely correct fat malabsorption in CF patients. OBJECTIVE The objective of the study was to compare the vitamin D status of children, adolescents, and young adults with CF who were treated with ro...

OBJECTIVE To compare sweat chloride values obtained by quantitative pilocarpine iontophoresis (classic test) with the sweat conductivity values obtained using Macroduct collection system in patients with and without cystic fibrosis (CF). The cost and time spent to carry out each test were also analyzed. METHODS The sweat test using both techniques was performed at the same time in patients wi...

The interfacial designing of carbon fiber (CF)/epoxy composites, as a well-accepted method used to obtain high-performance has gained extensive attention. However, an improvement in adhesion is usually accompanied by simultaneous decrease toughness, which become obstacle the performance enhancement CF/epoxy composites. Herein, nanosized UiO-66-NH2 was proposed yield on CF surface through situ g...