One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding facts that one-step qRT-PCR well suited diagnosis of and there are many commercially available kits in market, their high cost unavailability due to airport closures shipment restriction became a major bottleneck had driven desire produce ke...

Abstract Background Human noroviruses are one of the main causes foodborne illnesses and represent a serious public health concern. Rapid sensitive assays for human norovirus detection undoubtedly necessary clinical diagnosis, especially in regions without more sophisticated equipment. Method The rapid reverse transcription recombinase-aided amplification (RT-RAA) is fast, robust isothermal nuc...

Reverse transcription coupled with DNA amplification has become a well-established and powerful molecular technique for studying ribonucleic acids. However, the efficiency of those reactions is largely dependent on the molecular properties of currently used reverse transcriptases (RTs). Engineered and natural RT variants with improved thermostability and fidelity of DNA synthesis should be of g...

We amplified and sequenced the complete genome of enterovirus D68 (EV-D68) that had been collected from classroom air using a filter-based air sampling method and by swab sampling of environmental surfaces. Relatively high levels of EV-D68 genome equivalents were found per cubic meter of air by quantitative real-time reverse transcription-PCR (RT-PCR).

A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.

Pupply. With a reverse transcription-PCR (RT-PCR) amplifylng heat shock protein 70 (hsp70) gene, we were able to detect two differenttypes of viable Cyyptosporidium oocysts from the environment. Water specimens were filtered under positive pressure through a 3-Elm polycarbonate filter (Nuclepore filter, Whatman, Maidstone, England). The trapped materials were detached from the filter by sonicat...

Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...

enteroviruses are the causative agents of a number of diseases in humans. group b coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. genomic data of enteroviruses has allowed developing new molecular approaches such as nucleic acid sequence based amplification (nasba) for detection of such viruses. in this study, coxsackievirus b3 (cvb3) was de...