Objectives: There are many multifactorial causes for Congenital Heart Defects (CHDs) in which both genetic and non-genetic factors play role. MTHFD1 CBS two of the key enzymes that plays pivotal role metabolic pathway homocysteine. Most studies revealed genes involved folate/homocysteine pathways occurrence CHDs. The present study was planned to investigate common polymorphisms gene children wi...

The roots and stems of several Salacia species have been used as traditional medicines, especially in Ayurvedic medical system for the treatment diabetes, rheumatism, gonorrhea, amenorrhea, skin diseases, etc. Due to reported evidence supporting Salacia's beneficial effects early-stage diabetes other lifestyle-related Salacia-based dietary supplements health foods gaining popularity Japan count...

A composite genetic linkage map for the yellow fever mosquito Aedes aegypti was constructed based on restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and single strand conformation polymorphism (SSCP) markers. The map consists of 146 marker loci distributed across 205 cM, and includes several morphological mutant marker loci. Most of the genetic markers are ...

The aim of present study was to investigate myostatin gene polymorphism and its association with yearling weight records in Zel sheep using PCR-RFLP and PCR-SSCP methods. Blood samples were collected from 200 Zel sheep, randomly, and DNA was extracted using modified salting out method. Polymerase chain reaction was carried out to amplify 337, 222, and 311 bp fragments, respectively, comprising ...

Background: Lung cancer is the most common with 2,206,771 new cases in 2020 worldwide. MMP9 a member of matrix metalloproteinase family that also known as gelatinase B or IV type collagenase (92KD). through degrading Extracellular Matrix (ECM) and releasing growth factors has fundamental role tumorigenesis process. The C -1562 T SNP promoter increases expression susceptibility to lung cancer. T...

this study was performed to investigate two polymorphic sites from cyp19 gene (pvuii and mspi) and one polymorphic site from erα gene (snabi) in four cattle breeds including mazandarani, taleshi, sistani and simmental. in overall 278 samples for cyp19 and 206 samples for erα marker sites were genotyped using polymerase chain reactionsingle-strand conformation polymorphism (pcr-rflp) procedure. ...

Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...

A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110-PGRS (polymorphic GC-rich region)-PCR, also designated double-repetitive-element PCR (DRE-PCR). During the first phase of this investigation, 72 clinical strains representing 52 patients were initially typed by IS6110-r...

This study was performed to investigate two polymorphic sites from Cyp19 gene (PvuII and MspI) and one polymorphic site from ERα gene (SnaBI) in four cattle breeds including Mazandarani, Taleshi, Sistani and Simmental. In overall 278 samples for CYP19 and 206 samples for ERα marker sites were genotyped using polymerase chain reactionsingle-strand conformation polymorphism (PCR-RFLP) procedure. ...

Abstract Background It has been postulated that the interaction between environmental risk factors and genetic susceptibility is a possible cause for development of acute myeloid leukemia (AML). Cytochrome P450 (CYP) detoxification enzymes are responsible elimination oxidative stress. Genetic polymorphisms in these may AML due to enhanced accumulation reactive oxygen species. To study associati...