results of 45 patients, 4 (8.9% [95% ci: 4.4-15.6]) had detectable genomic hcv-rna in their pbmc specimens. hcv genotypes were determined in the pbmcs of these subjects showed that 2 (50.0%) subjects with occult hcv infection had hcv subtype 3a, and 2 (50.0%) had hcv subtype 1b. conclusions this study found that 8.9 % of the iranian candidates for liver transplant with cryptogenic cirrhosis had...

Die Bereiche RNA-Modifikation und RNA-Schaden weisen beide eine Vielzahl nicht-kanonischer Nukleosidstrukturen auf. Während sich RNA-Modifikationen zur Verbesserung der RNA-Funktion entwickelt haben, impliziert die Bezeichnung negative Auswirkungen. Auf Grundlage Markierung mit stabilen Isotopen Massenspektrometrie berichten wir von Identifizierung Charakterisierung 2-Methylthio-1,N6-ethenoaden...

During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...

In this study, freshwater fish Cyprinus carpio was exposed to sub lethal concentration of synthetic pyrethroids insecticide cypermethrin to evaluate the impact on nucleic acids in different organs. Fishes were exposed to sub lethal concentration (1/10th of LC50 value) of cypermethrin for three different durations, 7, 14 and 21 days. In seven day exposed fishes, DN...

background: in gene therapy, the use of rna molecules as therapeutic agents has shown advantages over plasmid dna, including higher levels of safety. however, transient nature of rna has been a major obstacle in application of rna in gene therapy. methods: here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3’ non-translated region of poliovirus to design an en...