The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...

this study was conducted to investigate performance of native chickens produced in the centre of production and breeding of native chicken of isfahan in rural areas of isfahan province by using 1440 native pullets with age of 45 days in 4 towns and 2 climate zone of warm and cold. this study employed by coworking 60 rural families that 24 pullets with age of 45 days with ratio of 5 hen to 1 roo...

DNA methylation is the major focus of studies on paternal epigenetic inheritance in mammals, but most previous studies about inheritable DNA methylation changes are passively induced by environmental factors. However, it is unclear whether the active changes mediated by variations in DNA methyltransferase activity are heritable. Here, we established human-derived DNMT3A (hDNMT3A) transgenic rat...

To determine the ME and amino acid digestibility of 5 soybean meal (SBM) samples, a precision-fed rooster assay and a chick assay were conducted. The 5 samples were cold-pressed (extruded) soybean meals or solvent-extracted (defatted) soybean meal. Of the cold-pressed varieties (unheated), there was an ultra-low trypsin SBM, a low-trypsin SBM, and a heated and unheated commodity SBM. The solven...

Nicotinamide phosphoribosyltransferase (NAMPT) is a cytokine hormone and rate-limiting enzyme involved in production of NAD and therefore affects a variety of cellular functions requiring NAD. Spermatogenesis and testicular steroidogenesis are likely to depend on NAD-dependent reactions and may therefore be affected by changes in testicular NAMPT expression. The objectives of the present study ...

Effect of Different Levels 2, 4 Dinitrophenol and Luteolin on Semen Quality Rooster during Freezing-Thawing Process

Ross, Martin R. (Connecticut State Department of Health, Hartford) and Earle K. Borman. Direct and indirect fluorescent-antibody techniques for the psittacosis-lymphogranuloma venereum-trachoma group of agents. J. Bacteriol. 85:851-858. 1963.-Direct and indirect fluorescent-antibody (FA) techniques were developed for the detection of group antigen in infected tissue cultures and the titration o...

By using a technique that previously demonstrated improved efficiency of intact crop removal for broilers, experiments were conducted to determine the effectiveness of the technique in 40and 41-wk-old broiler breeder roosters and hens. The technique involved (after scalding, defeathering, and head removal) separating (breaking) the neck from the carcass parallel with the shoulders and leaving t...

Extracellular superoxide dismutase (EC-SOD) is a metalloprotein and functions as an antioxidant enzyme. In this study, we used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. The recombinant lentiviruses were injected into the subgerminal cavity of freshly laid eggs. Subsequently, the embryos were incubated to hatch using phases II and III of the surrogate...

The role of growth hormone (GH) in amphibian metamorphosis is ambiguous based on experiments in which mammalian GH was administered to tadpoles and frogs. We have reexamined the effects of GH by producing transgenic Xenopus laevis that overexpress the cDNA encoding X. laevis GH. These transgenic tadpoles take the same length of time to reach metamorphosis as control tadpoles, but the transgenic...