The yeast splicing factor Cwc2 contacts several catalytically important RNA elements in the active spliceosome, suggesting that Cwc2 is involved in determining their spatial arrangement at the spliceosome's catalytic centre. We have determined the crystal structure of the Cwc2 functional core, revealing how a previously uncharacterized Torus domain, an RNA recognition motif (RRM) and a zinc fin...

The molecular mechanism for meiotic entry remains largely elusive in flowering plants. Only Arabidopsis SWI1/DYAD and maize AM1, both of which are the coiled-coil protein, are known to be required for the initiation of plant meiosis. The mechanism underlying the synchrony of male meiosis, characteristic to flowering plants, has also been unclear in the plant kingdom. In other eukaryotes, RNA-re...

Transcription termination of non-polyadenylated RNAs in Saccharomyces cerevisiae occurs through the action of the Nrd1-Nab3-Sen1 complex. Part of the decision to terminate via this pathway occurs via direct recognition of sequences within the nascent transcript by RNA recognition motifs (RRMs) within Nrd1 and Nab3. Here we present the 1.6 Å structure of Nab3-RRM bound to its UCUU recognition se...

The Relative Risk Model (RRM) is a tool used to calculate and assess the likelihood of effects to endpoints when multiple stressors occur in complex ecological systems. In this study, a Bayesian network was used to calculate relative risk and estimate uncertainty (BN-RRM) in the Puyallup River Watershed. First, we calculated the risk of prespawn mortality of coho salmon. Second, we evaluated th...

Est1 is a component of yeast telomerase, and est1 mutants have senescence and telomere loss phenotypes. The exact function of Est1 is not known, and it is not homologous to components of other telomerases. We previously showed that Est1 protein coimmunoprecipitates with Tlc1 (the telomerase RNA) as well as with telomerase activity. Est1 has homology to Ebs1, an uncharacterized yeast open readin...

This report investigates the possibility of using signal processing techniques in the analysis of biological sequences: DNA, RNA and proteins. The starting point in the investigation was the Resonant Recognition Model (RRM) and the method proposed here builds on the foundations set by RRM. However, some fundamental modi...cations to RRM had to be madein order to make it suitable for the analysi...

Wireless personal area networks (WPANs) will play an important role in next-generation communication networks. Currently, two technologies are being considered for the physical layer of WPANs, based on the two ultra wideband (UWB) standards, namely, multiband orthogonal frequency division multiplexing (MB-OFDM) UWB and direct-sequence (DS) UWB. The coexistence issue of these two types of WPANs ...

Proteins are the biomolecular workhorses driving the most biological processes in any living organism. These processes are based on selective interactions between particular proteins. So far, the rules governing the coding of the protein’s biological function, i.e. its ability to selectively interact with other biomolecules, have not been elucidated. The resonant recognition model (RRM) is a no...

The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA poly...

LRPPRC is a protein that has attracted interest both for its role in post-transcriptional regulation of mitochondrial gene expression and more recently because numerous mutated variants have been characterized as causing severe infantile mitochondrial neurodegeneration. LRPPRC belongs to the pentatricopeptide repeat (PPR) protein family, originally defined by their RNA binding capacity, and for...