The cause of type 1 diabetes continues to be a focus of investigation. Studies have revealed that interferon α (IFNα) in pancreatic islets after viral infection or treatment with double-stranded RNA (dsRNA), a mimic of viral infection, is associated with the onset of type 1 diabetes. However, how IFNα contributes to the onset of type 1 diabetes is obscure. In this study, we found that 2-5A-depe...

Ribonucleoproteins (RNP) are involved in many essential processes in life. However, the roles of RNA and protein subunits in an RNP complex are often hard to dissect. In many RNP complexes, including the ribosome and the Group II introns, one main function of the protein subunits is to facilitate RNA folding. However, in other systems, the protein subunits may perform additional functions, and ...

It has been postulated that retroviral recombination, like strong stop template switching, requires the RNase H activity of reverse transcriptase. To address this hypothesis, Moloney murine leukemia virus-based vectors, which were designed to test the recombination-related property of direct repeat deletion, were encapsidated in virions engineered to contain phenotypic mixtures of wild-type and...

Mao, G. 2018. Investigation of RNase P active site residues and catalytic domain interaction. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1623. 58 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0214-0. RNase P is an essential endoribonuclease responsible for the maturation of the tRNA 5’end. The RNase P family encompasses the...

Mercaptopurine and thioguanine are anticancer and immunosuppressive agents that exert their primary cytotoxic effects via incorporation of deoxythioguanosine (dG(s)) into DNA, but the precise mechanism(s) by which this causes cytotoxicity remains unknown. We initially determined that the level of dG(s) incorporation into DNA of human T- and B-lineage leukemia cell lines did not correlate signif...

A plate assay is described to detect extracellular RNase producing strains. RNA is used in the Petri dish assay for the determination of RNase activity. After growth of the organisms, the agar plate is flooded with a RNA precipitant and enzyme activity is shown by clear zones (halos) around the colonies. The simultaneous use of specific RNase inhibitor like diethyl pyrocarbonate allow the speci...

Self-incompatibility S-locus-encoded F-box (SLF) proteins have been identified in Antirrhinum and several Prunus species. Although they appear to play an important role in self-incompatible reaction, functional evidence is lacking. Here, we provide several lines of evidence directly implicating a role of AhSLF-S(2) in self-incompatibility in Antirrhinum. First, a nonallelic physical interaction...

background and objectives:purtscher's retinopathy occurs with sudden loss of vision. in this article, a case of post-partumpurtscher's-like retinopathy was investigated. case report:the patient was an 18-year-old woman who presented with severe bilateral loss of vision, three days after childbirth. no systemic abnormalities were observed except preeclampsia. the results of ophthalmosc...

Dimeric proteins can arise by the swapping of structural domains between monomers. The prevalence of this occurrence is unknown. Ribonuclease A (RNase A) is assumed to be a monomer near physiological conditions. Here, this hypothesis is tested and found to be imprecise. The two histidine residues (His12 and His119) in the active site of RNase A arise from two domains (S-peptide and S-protein) o...