introduction: the purpose of this study was formulation and preparation of a proper culture medium for saccharomyces cerevisiae var. boulardii with molasses and animal serum. methods: a fully-crossed factorial design contain 5%, 10% and 20% of molasses (m) with 0, 1% and 5% animal serum (s) was used in this study. the ph of all culture medias were adjusted to 5.6 with acetic acid. the seed was ...

Several yeast species, belonging to Saccharomyces and non-Saccharomyces genera, play fundamental roles during spontaneous must grape fermentation, and recent studies have shown that mixed fermentations, co-inoculated with S. cerevisiae and non-Saccharomyces strains, can improve wine organoleptic properties. During active dry yeast (ADY) production, antioxidant systems play an essential role in ...

Saccharomyces cerevisiae is an excellent model organism for addressing questions in cell biology, but other yeast systems are also providing new insights into several fundamental cellular processes.

This study investigated the effect of heat and ethanol on fermentable yeast cells. Fermentable yeast cells (Saccharomyces cerevisiae strains and non-saccharomyces species) were subjected to varying temperature (37, 40 and 44°C) and ethanol concentration (8, 10, 14 and 18%) respectively. In the research, 55.35% of the fermentable yeasts were highly thermotolerant, moderately thermotolerant (42.8...

Enabling technologies in synthetic biology now present the opportunity to engineer wine yeast for enhanced novel aromas. In doing so, improved products will increase desirability of consumer and add value winemaker. The action enzyme carotenoid cleavage dioxygenase 1 (CCD1) on ?-carotene produce ?-ionone is interest improve aroma flavour wine. Engineering yeast, Saccharomyces cerevisiae, higher...

The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is a scientific database for the molecular biology and genetics of the yeast Saccharomyces cerevisiae, which is commonly known as baker's or budding yeast. The information in SGD includes functional annotations, mapping and sequence information, protein domains and structure, expression data, mutant phenotypes, physical and gen...

The biosynthetic intermediate PRPP (phosphoribosylpyrophosphate) has a central role in cellular biochemistry since it links carbon and nitrogen metabolism. Its importance may be reflected in the fact that, in the Saccharomyces cerevisiae (yeast) genome, there are five unlinked genes, PRS1-PRS5, each of which is theoretically capable of encoding the enzyme synthesizing PRPP. Interference with th...

Studies conducted in the early 1990s showed for the first time that Saccharomyces cerevisiae can undergo cell death with hallmarks of animal apoptosis. These findings came as a surprise, since suicide machinery was unexpected in unicellular organisms. Today, apoptosis in yeast is well-documented. Apoptotic death of yeast cells has been described under various conditions and S. cerevisiae homolo...

The role of the yeast vacuole, a functional analogue of the mammalian lysosome, in the turnover of proteins and organelles has been well documented. This review provides an overview of the current knowledge of vesicle mediated vacuolar transport in the yeast Saccharomyces cerevisiae cells. Due to the conservation of the molecular transport machinery S. cerevisiae has become an important model s...

The increasing need for alternative fuels to replace fossil has made bioethanol a promising option. Although numerous sources of sugar generation and agricultural wastes can be converted into ethanol, Avocado Seeds (AS) are particularly attractive as raw materials due their abundance, high carbohydrate content, lack interactions with the food chain. Therefore, this study investigated potential ...