A modular approach has been developed for enzymatic ligation of peptides/proteins with oligonucleotides through the design tag phosphoramidites as adaptors, paving way towards streamlined production peptide/protein-oligonucleotide conjugates.

The soluble proteins in the hemolymph, the salivary gland, and the salivary secretion of fourth instar Chironomus tentans were examined by disc electrophoresis in acrylamide gels. Of the 11 protein fractions detected in buffered saline extracts of the gland, 10 are present also in the hemolymph. Amino acid isotope incorporation experiments indicate that the protein fractions shared by the saliv...

Liquid-liquid phase separation (LLPS) of proteins mediates the assembly biomolecular condensates involved in physiological and pathological processes. Identifying minimalistic building blocks sequence determinant protein is an urgent need but remains challenging partially due to lack methodologies characterize behavior. Here, we demonstrate computational tools efficiently quantify microscopic f...

In molecular radiotherapy with 177Lu-labeled prostate specific membrane antigen (PSMA) peptides, kidney and/or salivary glands doses limit the activity which can be administered. The aim of this work was to investigate the effect of the ligand amount and injected activity on the tumor-to-normal tissue biologically effective dose (BED) ratio for 177Lu-labeled PSMA peptides. For this retrospectiv...

The secreted salivary proteins from two cereal aphid species, Sitobion avenae and Metopolophium dirhodum, were collected from artificial diets and analysed by tandem mass spectrometry. Protein identification was performed by searching MS data against the official protein set from the current pea aphid (Acyrthosiphon pisum) genome assembly and revealed 12 and 7 proteins in the saliva of S. avena...

ABSTRACT Lena Ho studied biochemistry at the University of Wisconsin–Madison, USA, funded by a scholarship from Singapore Economic Development Board. then joined lab Gerald Crabtree Stanford for her PhD, where she investigated function and mechanism chromatin remodelling proteins in epigenetic regulation embryonic, haematopoietic cancer stem cells. She moved back to postdoc Bruno Reversade's In...

Bioactive peptides (BPs) are multifunctional proteins that have a variety of activities and strong pharmacological effects. Due to the food-derived BPs' extensive spectrum health benefits numerous bioactivities, including antioxidant, immunological, anti-ageing, anti-inflammatory activities, research on these compounds has not been interrupted throughout past few decades. To balance daily rise ...

The effect of salivary amylase on changes in gastric protein hydrolysis was studied. study carried out vitro, saliva, juice, solutions starch, casein, albumin and hemoglobin were used the work. It concluded that use starch-protein mixtures helps to reduce by due formation complexes prevent hydrolysis, access proteases proteins complex. An increase ratio starch direction increasing contributes a...