Specific pathogen-free CD-1 and C57Bl mice were infected in a hind footpad with 5 x 10(4) viable Salmonella enteritidis cells or 10(7) viable S. pullorum cells. The resulting bacterial growth within the footpad, the draining lymph nodes, and the liver and spleen was followed for 14 days. Mice vaccinated with live S. enteritidis rapidly developed an effective antibacterial resistance to both int...

Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated...

While real-time-polymerase chain reaction (RT PCR) has been used as a rapid test for detection of Salmonella Enteritidis in recent years, little research has been done to assess the feasibility of pooling poultry environmental samples with a Salmonella Enteritidis-specific RT PCR assay. Therefore the objective of this study was to compare RT PCR Salmonella Enteritidis detection in individual an...

Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmo...

OBJECTIVES To determine the current state of antimicrobial resistance among non-typhoidal Salmonella strains isolated from humans in Spain. METHODS All strains of Salmonella from human sources received in the reference laboratory from 2001 to 2003 were serotyped and phage types were determined in the most common serovars. A systematic sampling procedure was carried out in order to obtain a ra...

The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochl...

BACKGROUND AND PURPOSE Group D non-typhoidal Salmonella infection is increasing in Taiwan. This study aimed to investigate the changing serotypes and antibiotic resistance of childhood group D Salmonella infection. METHODS From 1998 through 2004, children (<16 years) infected with group D Salmonella were retrospectively reviewed. Demographic data, clinical and laboratory features, and risk fa...

Methods The PCR-based detection technique for five Salmonella species was tested under O.I.E. requirements, to determine specificity, sensitivity, and repeatability. Primers were calculated by OLIGO software and produced with HPLC purification. Amplification was conducted by a T3000 Biometra PCR-machine. Testing was performed on a panel of five basic agents: Salmonella enterica ser. Enteritidis...

Salmonella enterica serovar Enteritidis are facultative intracellular bacteria that cause disease in numerous species. Salmonella-related infections originating from poultry and/or poultry products are a major cause of human foodborne illness with S. Enteritidis the leading cause worldwide. Despite the importance of Salmonella to human health and chickens being a reservoir, little is known of t...

Cryogenic cooling of shell eggs with carbon dioxide (CO(2)) is known to improve egg content quality through rapid cooling as well as by increasing internal CO(2) levels. A study was undertaken to determine the effects of variations in atmospheric CO(2) concentrations (aerobically stored, flushed with CO(2) and sealed, or bubbled with CO(2)) on the survival and growth of Salmonella Enteritidis i...