Sunflowers are among the world’s most widely cultivated oilseeds with an interesting nutritional composition. A biomass composed mainly of carbohydrates, fibers, and proteins is generated from sunflower oil production. In this context, objective study was to investigate application emerging technologies obtain edible protein-rich flour potential be exploited in food industry. The effects optimi...

Transposon mutagenesis of Bordetella pertussis was used to discover mutations in the cytochrome c biogenesis pathway called system II. Using a tetramethyl-p-phenylenediamine cytochrome c oxidase screen, 27 oxidase-negative mutants were isolated and characterized. Nine mutants were still able to synthesize c-type cytochromes and possessed insertions in the genes for cytochrome c oxidase subunits...

IMPORTANCE Mitochondrial disorders have emerged as a common cause of inherited disease, but their diagnosis remains challenging. Multiple respiratory chain complex defects are particularly difficult to diagnose at the molecular level because of the massive number of nuclear genes potentially involved in intramitochondrial protein synthesis, with many not yet linked to human disease. OBJECTIVE...

Copper homoeostasis was investigated in the Drosophila melanogaster S2 cell line to develop an insect model for the study of copper regulation. Real-time PCR studies have demonstrated expression in S2 cells of putative orthologues of human Cu regulatory genes involved in the uptake, transport, sequestration and efflux of Cu. Drosophila orthologues of the mammalian Cu chaperones, ATOX1 (a human ...

Deficiencies in the activity of cytochrome c oxidase (COX), the terminal enzyme in the respiratory chain, are a frequent cause of autosomal recessive mitochondrial disease in infants. These patients are clinically and genetically heterogeneous, and all defects so far identified in this group have been found in genes coding for accessory proteins that play important roles in the assembly of the ...

Deficiencies in the activity of cytochrome c oxidase (COX) are an important cause of autosomal recessive respiratory chain disorders. Patients with isolated COX deficiency are clinically and genetically heterogeneous, and mutations in several different assembly factors have been found to cause specific clinical phenotypes. Two of the most common clinical presentations, Leigh Syndrome and hypert...

Photoelectron spectra of early 3d-transition metal dioxide anions, ScO2−, TiO2−, VO2−, CrO2−, and MnO2−, are calculated using semilocal hybrid density functional theory (DFT) many-body perturbation within the GW approximation one-shot perturbative eigenvalue self-consistent formalisms. Different levels compared with each other available photoelectron spectra. We show that a PBE0 starting point ...

Abstract Within this study, the blade shape of a large-scale axial turbine operating with sCO2 blended dopants is optimized using an integrated aerodynamic-structural three-dimensional (3D) numerical model, whereby optimization aims at maximizing aerodynamic efficiency whilst meeting set stress constraints to ensure safe operation. Specifically, three candidate mixtures are considered, namely, ...