Aging is associated with a dramatic decrease in sleep intensity and continuity. The selective GABA(A) receptor agonist gaboxadol has been shown to increase non-REM sleep and the duration of the non-REM episodes in rats and sleep efficiency in young subjects and to enhance low-frequency activity in the electroencephalogram (EEG) within non-REM sleep in both rats and humans. In this double-blind,...

Thirteen Targhee rams selected for rate and efficiency of gain for 4 yr (1.5 generations) were compared with 10 rams from a Targhee line with no selection for over 20 yr to determine if selection for these traits would be associated with changes in the secretion of growth hormone (GH), thyrotropin (TSH) and(or) prolactin (PRL). Selected rams exhibited greater birth weight, average daily gain (A...

BACKGROUND Clearing subglottic secretions has been proved to reduce ventilator-associated pneumonia. A manual method named the rapid flow expulsion maneuver was developed to clear subglottic secretions. This study evaluates its safety and effectiveness and analyzes the influential factors. METHODS This study was composed of 2 parts. In an in vitro study, secretions were instilled directly to ...

Somatic cell nuclear transfer (SCNT) is a useful tool for animal cloning, but the efficiency of producing viable offspring by SCNT is very low. To improve this efficiency in the production of cloned pigs, it is critical to understand the interactions between uterine function and cloned embryos during implantation. Lysophosphatidic acid (LPA) is a lipid mediator that plays an important role in t...

not affect glucose-stimulated insulin secretion (GSIS) and does not protect against the harmful effects of palmitate. We have shown that palmitateinduced GSIS impairment is linked to attenuation of glucose sensitivity of mitochondrial respiration and dampening of the coupling efficiency of glucose-stimulatedoxidative phosphorylation [1]. Since these bioenergetic parameters are affected similarl...

Target directed proteolysis allows specific processing of proteins in vivo. This method uses tobacco etch virus (TEV) NIa protease that recognizes a seven-residue consensus sequence. Because of its specificity, proteins engineered to contain a cleavage site are proteolysed, whereas other proteins remain unaffected. Therefore, this approach can be used to study the structure and function of targ...

VirB5 is a type 4 secretion system protein of Agrobacterium located on the surface of the bacterial cell. This localization pattern suggests a function for VirB5 which is beyond its known role in biogenesis and/or stabilization of the T-pilus and which may involve early interactions between Agrobacterium and the host cell. Here, we identify VirB5 as the first Agrobacterium virulence protein tha...