BACKGROUND Although ovarian cryopreservation in patients with cancer should ideally be performed before the initiation of therapy, cryopreservation from such patients often becomes an option only later. The justification for the procedure needs to be elucidated. METHODS Eighteen cancer patients before chemotherapy and 23 others after chemotherapy participated in the study. Freshly dissected o...

Recent work in preservation of female fertility as well as new information on the nature of spermatogonial stem cells has prompted an investigation into the possibility of an effective clinical-grade procedure for the cryopreservation of testicular cells and/or tissue. Clinical-grade reagents, validated equipment, and protocols consistent with cGTP/cGMP standards were used in developing a proce...

We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2-1159 days) from patients of varying ages (26-62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured a...

Despite extensive experimental work, neither the effect of long term cryopreservation on vein graft archi tecture nor the failure of alloveins due to graft rejection have yet been investigated. Herein, we investigated ul trastructurally: a) the integrity of rabbit jugular veins following 1, 2 and 3 months of cryopreservation; b) the outcome of the three-month cryopreserved vein autoand allograf...

Previous studies indicated the existence of some amount of variability in the embryonic development success of cryopreserved sea-urchin (Paracentrotus lividus) embryos. The present work studied the covariation of cryopreservation results fluctuations and reproductive parameters (condition index, CI and oocytes quality) during the reproductive season, in order to verify the hypothesis of the eff...

* Author for correspondence:Research Scientist, Conservation & Biotechnology, (Visiting Research Fellow in Conservation & Molecular Genetics, University of Derby), Damar, Drum Road, Cuparmuir, Cupar, Fife, KY15 5RJ, Scotland, UK. Tel. and Fax: 00 44 (0) 1334 657192, Email: [email protected]. Abstract. Cryopreservation plays a vital role in the long-term in vitro conservation of esse...

Single cells suspensions used for grafting in the clinical setting may be reliably cryopreserved by established protocols. However, for tissue engineered constructs which now also get used as grafts in the clinic such established protocols and assays which indicate graft viability and their function as graft do not exist. a) The purpose was to develop a cryoprotocol and an animal model to test ...

A bone marrow transplant (BMT) is one kind of standard treatment modality in advanced hemato-oncology. In order to set up a BMT unit, one of the important steps before starting a clinical program is to evaluate the cryopreservation procedure for stem cell storage. Twenty one bags of buffy coat were used to be the testing specimens. They were processed and frozen according to cryopreservation pr...

Cryopreservation of plant tissues in liquid nitrogen is now used for long-term conservation of vegetatively-propagated crops. Development of standard techniques for cryopreservation is important to the international plant-conservation community for successful implementation of storage protocols in diverse and internationally dispersed laboratories. Evaluation of the critical points of each pres...

UNLABELLED While cocoa plants regenerated from cryopreserved somatic embryos can demonstrate high levels of phenotypic variability, little is known about the sources of the observed variability. Previous studies have shown that the encapsulation-dehydration cryopreservation methodology imposes no significant extra mutational load since embryos carrying high levels of genetic variability are sel...