DeLeve, Laurie D., Xiangdong Wang, Margaret K. McCuskey, and Robert S. McCuskey. Rat liver endothelial cells isolated by anti-CD31 immunomagnetic separation lack fenestrae and sieve plates. Am J Physiol Gastrointest Liver Physiol 291: G1187–G1189, 2006. First published June 15, 2006; doi:10.1152/ajpgi.00229.2006.— The gold standard for the identification of sinusoidal endothelial cells (SEC) is...

The resolution of separation two elementary signals forming a partially coherent superposition, defined by quantum Fisher information and normalized with respect to detection probabilities, is always limited the incoherent mixtures. However, when superpositions are prepared in controlled way, precision can be enhanced up several orders magnitude above this limit. Coherence also allows sorting a...

PURPOSE It is still technically difficult to collect high purity cancer cells from tumor tissues, which contain noncancerous cells. We hypothesized that xenograft models of NOG mice expressing enhanced green fluorescent protein (eGFP), referred to as NOG-EGFP mice, may be useful for obtaining such high purity cancer cells for detailed molecular and cellular analyses. METHODS Pancreato-biliary...

Neurons are highly polarized cells that require continuous turnover of membrane proteins at axon terminals to develop, function, and survive. Yet, it is still unclear whether membrane protein degradation requires transport back to the cell body or whether degradation also occurs locally at the axon terminal, where live observation of sorting and degradation has remained a challenge. Here, we re...

BACKGROUND The throughput of electrophysiological recording is growing rapidly, allowing thousands of simultaneous channels, and there is a growing variety of spike sorting algorithms designed to extract neural firing events from such data. This creates an urgent need for standardized, automatic evaluation of the quality of neural units output by such algorithms. NEW METHOD We introduce a sui...

A size-dependent particle separation (classification) is one of the most important procedures in biochemical, environmental, or medical analyses and their applications. However, the smaller particle size or the smaller size difference makes the particle classification difficult. The Lab-on-a-Chip concept have encouraged the miniaturization of various separation or selection methods for small pa...

To study the electrophysiological properties of neuronal networks, in vitro studies based on microelectrode arrays have become a viable tool for analysis. Although in constant progress, a challenging task still remains in this area: the development of an efficient spike sorting algorithm that allows an accurate signal analysis at the single-cell level. Most sorting algorithms currently availabl...

Synchronous spike discharge of cortical neurons is thought to be a fingerprint of neuronal cooperativity. Because neighboring neurons are more densely connected to one another than neurons that are located further apart, near-synchronous spike discharge can be expected to be prevalent and it might provide an important basis for cortical computations. Using microelectrodes to record local groups...

The ability to build and control complex biological systems is greatly enhanced by the generation of related parts with varying strengths. In this way, various parts can be strung together and the connectivity and expression levels can be matched for the desired system performance. Engineered gene circuits, both in vivo and in vitro, often utilize the T7 RNA polymerase in tandem with the T7 pro...