A liquid chromatographic-mass spectrometric method for the determination of cortisol in serum using atmospheric pressure electrospray ionization and tandem mass spectrometry is described. During sample preparation, 150 microl of serum were deproteinized with methanol/zinc sulfate followed by on-line solid phase extraction employing column switching. Tri-deuterated cortisol was used as the inter...

it has been suggested that ghrelin may play a role in the response of growing hormone (gh) to exercises. the present study was designed to examine the effects of 6 weeks of wrestling and a wrestling–based circuit exercise (wbce) on plasma ghrelin and some glucoregulatory hormones in well-trained wrestlers. 30 well-trained male wrestlers (age 18.5±1.5 and bmi 24.8±6.81) voluntarily participated ...

The performance characteristics are reported for a serum cortisol assay using the GAMMAFLO Automated Assay System, an automated continuous-flow system for radioimmunoassay (RIA). This automated system utilizes a combination of continuous-flow methodology and column chromatography techniques. The cortisol assay studied had a standard curve range of 10.0 to 500 microgram per L. The intra-assay an...

To determine the effect of long-distance (approximately 600 km) road transportation on the blood biochemistry of laboratory animals, we investigated the changes in serum biochemical parameters in healthy cynomolgus monkeys and beagle dogs transported by truck from Osaka to Tsukuba, Japan. The concentrations of serum cortisol, total bilirubin and aspartate aminotransferase in monkeys increased d...

Adrenocorticotropic hormone (ACTH) challenge test is recognized as a method for evaluating some forms of stress. Six lacting cows, well trained to blood sampling were used for this study. Cows were randomly assigned to receive saline or an intramuscular single dose (0.5 μg/kg) of ACTH (Synacthen Depot). Blood samples (10 mL) were collected from the coccygeal blood vessels of all cows at 0 h (im...

To assess whether differences in the adrenal response to allergen are important in determining the magnitude of the allergen induced late responses in asthmatic subjects, we measured serum cortisol concentrations after inhalation challenge with allergen or control solution (phosphate buffered saline). The two challenges were performed in random order with an interval of 14 days. A normal diurna...

In the present study tissue cholesterol and serum cortisol changes during two reproductive phases have been correlated in the freshwater fish Notopterus notopterus. The reproductive cycle of N. notopterus has two phases such as breeding phase (April - August) and non breading phase (October - December). The cholesterol content of the ovary and liver increased during breeding phase. The serum co...