Nodal explants bearing a single axillary bud, from three cultivars of cultivated grape, viz., `Thompson seedless', `Sonaka' and `Tas-e-Ganesh', were used to initiate shoot cultures on G16 medium containing adenine sulphate, monobasic sodium phosphate, BAP and NAA. Each shoot, developed from an axillary bud, produced a tuft of multiple shoots on a medium containing BAP, calcium pantothenate, mon...

The frequency of shoot regeneration and multiplication of P. harmala was influenced by the type of explant and kind and concentration of hormones. Of the various seedling explants, cotyledonary node exhibited maximum shoot regeneration frequency from axillary region on MS medium supplemented with 5 microM BAP. Addition of 0.1 microM NAA enhanced the efficacy of BAP for multiple shoot regenerati...

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one these species, the region for traditional medicine ornamental purposes. Therefore, optimizing alternative micropropagation protocols tissue culture conditions secondary metabolite production needs attention. effect Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot r...

Wild plant species are of great interest as a source pharmacologically valuable compounds but number them endemic and/or endangered ones. Modern biotechnology can provide reliable methods for their utilization without disturbing natural populations. In vitro culture Rhodiola being intensively developed to include into various biotechnological programmes. Aim. Development protocol direct rosea L...

Micropropagation of three Echinacea species, E. angustifolia DC., E. pallida Nutt., and E. purpurea Moench., was investigated as a potential means of germplasm preservation of species faced with overcollection in the wild and rapid clonal propagation of elite individuals with unique medicinal or ornamental properties. Very high contamination rates occurred with shoot-tip explants but not with n...