Simple sequence repeats (SSRs) are widely used in mapping constructions and comparative genetic diversity analyses. Here, 103,056 SSR loci were found Cucurbita species by silico PCR. In general, the frequency of these SSRs decreased with increase motif length, di-nucleotide motifs most common type. For same repeat types, sharply number. The majority suitable for marker development (84.75% mosch...

Many new classes of Polymerase Chain Reaction (PCR) -based genetic markers have been developed over the last decade. Of these, microsatellite markers are widely regarded as one of the most useful identifi ed so far1,2,3. The signifi cance of microsatellite markers derives from their abundance in the genome, single locus nature, simplicity of assay, high levels of allelic diversity, mendelian in...

micropropagated plantlets derived from three different grape rootstock genotypes namely, dogridge (vitis champini), so4 (v. berlandieri×v. rupestris) and ari-h-144 (v. vinifera×v. labrusca) were subjected to randomly amplified polymorphic dna (rapd) and inter simple sequence repeats (issr) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...