In this paper, we report on demonstration of digital counting of enzyme based on fluorescent femtoliter droplet array imaging by using a lensless CMOS image sensor. The result supports the device can be applied to digital enzyme linked immunosorbent assay (ELISA). Digital ELISA is an ultra-sensitive detection technique for a biomarker [1, 2]. In the previous work, we developed a miniature lensl...

We automate the manipulation of genomic-length DNA in a nanofluidic device based on real-time analysis of fluorescence images. In our protocol, individual molecules are picked from a microchannel and stretched with pN forces using pressure driven flows. The millimeter-long DNA fragments free flowing in micro- and nanofluidics emit low fluorescence and change shape, thus challenging the image an...

In this paper, we present a novel cell counting method accomplished using a single-cell array fabricated on an image sensor, complementary metal oxide semiconductor sensor. The single-cell array was constructed using a microcavity array, which can trap up to 7,500 single cells on microcavities periodically arranged on a plane metallic substrate via the application of a negative pressure. The pr...

While conventional MRI scanners rely on large magnets, extremely homogeneous and increasingly strong polarizing fields, recent years have also seen the development of small, single-sided NMR systems suitable for a variety of applications in the realm of biomedicine, such as tendon and skin [1]. These novel imaging systems are typified by their small size, single-sided nature and nonuniform pola...

Single-molecule manipulation instruments have unparalleled abilities to interrogate the structure and elasticity of single biomolecules. Key insights are derived by measuring the system response in varying solution conditions; yet, typical solution control strategies require imposing a direct fluid flow on the measured biomolecule that perturbs the high-sensitivity measurement and/or removes in...

Digital microfluidics is a popular tool for lab-on-a-chip applications and is typically implemented in one of two formats: single-plate “open” devices or two-plate “closed” devices. Single-plate devices have some advantages relative to the more common two-plate format such as faster mixing, the capacity to move larger volumes on a given footprint, and easier access to droplets for handling or o...

The goal of this research was to develop a chemical gas sensing device based on single-walled carbon nanotube (SWCNT) networks. The SWCNT networks are synthesized on Al(2)O(3)-deposted SiO(2)/Si substrates with 10 nm-thick Fe as the catalyst precursor layer using microwave plasma chemical vapor deposition (MPCVD). The development of interconnected SWCNT networks can be exploited to recognize th...

AIMS We report a single-centre experience of extraction of infected pacing and ICD leads. METHODS Retrospective study of consecutive lead extractions for infection. Lead extraction was by traction, or, if unsuccessful, a laser sheath was used. All procedures were performed in the operating room. RESULTS Leads were extracted in 82 patients including 66 patients (80%) with infection occurring...

We present a novel concept to process human blood on a spinning polymer disk for the determination of the hematocrit level by simple visual inspection. The microfluidic disk which is spun by a macroscopic drive unit features an upstream metering structure and a downstream blind channel where the centrifugally enforced sedimentation of the blood is performed. The bubble-free priming of the blind...

This paper introduces a device, algorithm and graphical user interface to obtain anthropometric measurements of foot. Presented device facilitates obtaining scale of image and image processing by taking one image from side foot and underfoot simultaneously. Introduced image processing algorithm minimizes a noise criterion, which is suitable for object detection in single object images and outpe...