background: serological assay based on dense granular (gra) proteins of toxoplasma gondii (t. gondii) is actually the most popular laboratory diagnostic tool to detection of toxoplasmosis. we aimed to construct a recombinant gra7-ptz57rt plasmid vectors that it is suitable for sub-cloning and gra7 protein production. m aterials and methods: souris mice were used for maintaining of t. gondii tac...

objective: whey protein is a byproduct of dairy industry, where cheese and casein are manufactured.this is the major protein content which is loss during the formation of yogurt and cheese. methods: if this is added to the diet then that can enhance the nutritioal value for the diet, which will be very usefull. results: in this study we try to find it out that the whey protein has the antibioti...

abstract   background : cancer has not been elucidated in colerectal site. c-reactive protein (crp) is a product synthesized in hepatocytes and has been reported to be up-regulated by such proinflammatory cytokines as interleukin-1 (il-1), interleukin-6 (il-6), and tumor necrosis factor (tnf). the significance of a preoperative serum elevation was evaluated using crp as a predictive indicator f...

The surface (SU) envelope glycoproteins of feline leukemia virus subgroup B (FeLV-B) and amphotropic murine leukemia virus (A-MLV) are highly related, even in the variable regions VRA and VRB that have been shown to be required for receptor recognition. However, FeLV-B and A-MLV use different sodium-dependent phosphate symporters, Pit1 and Pit2, respectively, as receptors for infection. Pit1 an...

Expression of the transmembrane receptor protein Ram-1 may be critical to optimizing retroviral gene transfer. Ram-1 acts as both a sodium-dependent phosphate transporter and a receptor for amphotropic retroviruses. We previously reported detectable Ram-1 in murine hematopoietic fetal liver cells (FLC) despite resistance of these cells to amphotropic retroviral transduction (infection). We docu...

Plants are often subjected to several environmental stresses throughout their life cycle. Adaptation of plants to saline conditions may be due to some salt-related changes in the pattern of gene(s) expression. The physiological role of stress induced proteins is not yet clearly understood. However, it is believed that these proteins allow plants to produce biochemical and structural adjustments...

congenital recessive myotonia is a rare genetic disorder caused by mutations in clcn1, which codes for the main skeletal muscle chloride channel clc-1. more than 120 mutations have been found in this gene. the main feature of this disorder is muscle membrane hyperexcitability. here, we report a 59-year male patient suffering from congenital myotonia. he had transient generalized myotonia, which...

background toxoplasma gondii is an obligatory intracellular protozoan parasite, which infects human beings. since the current antigens used for diagnosis or vaccination are contaminated with non parasitic material in which the parasite is grown, it is tried to produce recombinant antigens to design vaccines against toxoplasmosis, or make diagnostic kits. choosing the type of antigen to produce ...

background: the over-expression of recombinant proteins in large amount is important for production of therapeutic proteins and structural study. there are several systems for expression of recombinant proteins. one of the most relevant expression systems is escherichia coli (e. coli). although this organism has many advantages, most of recombinant proteins expressed in e. coli hosts form inclu...