objective: the aim of the current study was to examine the effectiveness of barkley's parent training program, working memory training and the combination of these two interventions for children with attention deficit hyperactivity disorder (adhd). methods: in this study, 36 participants with adhd (aged 6 to 12 years) were selected by convenience sampling. revision of the swanson, nolan and pel...

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak inte...

Background: Phospho-modulation of SNARE function has not yet known. Results: Phospho-SNAP-25 was de-phosphorylated by protein phosphatase-1 whose activity was regulated by PRIP, thus regulating exocytosis. Conclusion: Protein phosphatase-1 whose activity is regulated by PRIP, is the major phosphatase responsible for the de-phosphorylation of SNAP-25. Significance: The results provide the first ...

We examined whether cGMP-dependent protein kinase (PKG) and mitochondrial ATP-sensitive potassium (K(ATP)) channels are involved in S-nitroso-N-acetyl penicillamine (SNAP)-induced reactive oxygen species (ROS) generation. SNAP significantly increased ROS generation in cardiomyocytes. This increase was suppressed by both 5-hydroxydecanoate (5-HD) and glibenclamide. Direct opening of mitochondria...

cDNA clones of a neuronal-specific mRNA encoding a novel 25-kD synaptosomal protein, SNAP-25, that is widely, but differentially expressed by diverse neuronal subpopulations of the mammalian nervous system have been isolated and characterized. The sequence of the SNAP-25 cDNA revealed a single open reading frame that encodes a primary translation product of 206 amino acids. Antisera elicited ag...

Synchronization of neurotransmitter release with the presynaptic action potential is essential for maintaining fidelity of information transfer in the central nervous system. However, synchronous release is frequently accompanied by an asynchronous release component that builds up during repetitive stimulation, and can even play a dominant role in some synapses. Here, we show that substitution ...

Nitric oxide (NO) has many important physiological functions, including its ability to inhibit platelet activation and serve as potent antimicrobial agent. The multiple roles of NO in vivo have led to great interest in the development of biomaterials that can deliver NO for specific biomedical applications. Herein, we report a simple solvent impregnation technique to incorporate a nontoxic NO d...

The SNARE proteins syntaxin, SNAP-25, and synaptobrevin play a central role during Ca(2+)-dependent exocytosis at the nerve terminal. Whereas syntaxin and SNAP-25 are located in the plasma membrane, synaptobrevin resides in the membrane of synaptic vesicles. It is thought that gradual assembly of these proteins into a membrane-bridging ternary SNARE complex ultimately leads to membrane fusion. ...

ObjectiveProvide the first baseline census of Supplemental Nutrition Assistance Program Education (SNAP-Ed) state implementing agencies’ (SIAs) intent to use and evaluate 51 indicators described in SNAP-Ed Evaluation Framework.MethodsA cross-sectional study design was used administer electronic surveys 124 SIAs who received funding 2017. Descriptive statistics were analyze results.ResultsOf ind...