Peptide sera specific for the C, C/C', and P proteins of Sendai virus have been used to confirm that the viral nonstructural proteins originate from internal AUG codons and are translated in a different reading frame from that of the P protein. The C protein undergoes aberrant migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and is expressed at higher levels in infected ce...

Arginase (EC 3.5.3.1) was purified to homogeneity from cytosol of soybean, Glycine max, axes by chromatographic separations on Sephadex G-200, DEAE-sephacel, hydroxyapatite, and arginine-affinity columns. The molecular weight of the enzyme estimated by pore gradient gel electrophoresis was 240,000, while sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecul...

Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S]methionine and [3H]uridine. At least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was det...

Abbreviations: CAPS: N-cyclohexyl-3-aminopropanesulfonic acid cDNA: omplementary DNA to mRNA CHES: N-cyclohexyl-2-aminoethanesulfonic acid. DTT: dithiothreitol FPLC: fast protein liquid chromatography IPTG: isopropylthio-β-D-galactoside LB: Luria-Bertani MES: 2-(N-morpholino)ethanesulfonic acid Ni-NTA: Nickel-nitriloacetic acid ORF: open reading frame PCR: polymerase chain reaction PMSF: phenyl...

The membrane proteins of erythrocytes were labeled by injecting L-[14C]-leucine and later L-[3H]leucine into rats, the two injections being 31 days apart. Control animals received the two isotopic forms of L-leucine simultaneously. Deviations in labeling ratio from control patterns were found on sodium dodecyl sulfate-polyacrylamide gel electrophorograms in restricted regions suggestive of turn...

It was confirmed that the thiol protease inhibitory activities in human lung cancer extracts were significantly higher than normal lung tissue extracts. Furthermore the thiol protease inhibitor was extracted from human lung cancer tissue and purified by papain-Sepharose and Sephadex G-100 column chromatographies. The purified inhibitor inhibited papain and ficin, and its molecular weight determ...

Interferon produced by human diploid fibroblast cells in culture has been purified approximately 5000-fold. The purified interferon, when analyzed by electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate, contains only one polypeptide component of 20,000 molecular weight. The interferon activity comigrates with this polypeptide, indicating identity of the activity and the poly...

Polyacrylamide Gel Electrophoresis (PAGE) is both an analytical and preparative tool widely used in protein analysis. Proteins are separated by PAGE in an electric field according to their size, shape and charge both in their native configuration (native PAGE) or in the presence of denaturing agents such as the ionic detergent sodium dodecyl sulfate (SDS-PAGE). A third type of electrophoretic t...

Incubation of UDP-N-acetyl[3H]glucosamine with purified inner membrane preparations of a mutant (UDPgalactose-4-epimeraseless and glucosamine-6-phosphate synthetaseless) Escherichiu coli 0111-B4 results in the incorporation of N-acetyl[3H]glucosamine into two components, A and B, that may be distinguished by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. A contain...

Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of nuclear non-histone proteins extracted with 0.15 and 0.30 M NaCl from human normal lymphocytes or leukemic leukocytes indicated a lack of bands in the highermolecular-weight regions of the gels. However, the corre sponding fractions from phytohemagglutinin-stimulated lymphocytes and Burkitt lymphoma cells did contain highmolecul...