expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

in line with an investigation regarding to evaluation of the compatibility of the asian pear cultivars with iran climatic conditions – currently being carried out at the horticultural department of tarbiyat modares university (tmu) – the present preliminary study was performed in two regions in isfahan. asian pear (pyrus serotina rehd.) cultivars (grafted on european pear seedling rootstocks) w...

Freezing injury is an important limiting factor in the production of pomegranate in Iran.The aim of the present study was to evaluate the effect of paclobutrazol (PBZ) on cold hardiness of pomegranate (Punica granatum L.) cv. ‘Malas Saveh’. Different concentrations of PBZ including 0 (control), 31, 62, 125 or 250 mg L–1 were sprayed on one-year-old plants in August and the electrolyte leakage o...

      A major hurdle in pharmaceutical formulation is water insolubility of most of drugs affecting their stability and bioavailability. If the drug is also insoluble in organic medium, it is difficult to deliver it in a sufficiently bioavailable form and hence it is a great challenge to formulation researchers to overcome such difficulty. Although some approaches are available for enhancing th...

Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...

in order to study the canopy temperature effect on protein and soluble sugar, oil percentage and yield of soybean in different planting dates, an experiment was conducted as factorial based on randomized complete block design with three replications in karaj and moghan areas during 2012-13. the studied treatments were included cultivar (‘williams’ and ‘l17’) and planting date (5th of may, 5th o...