A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...

The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, respectively, as a substrate. The particles exert their effect by binding soluble...

An assessment of elastase-substrate kinetics and adsorption at the solid-liquid interface of peptide-bound resin was made in an approach to the solid-phase detection of human neutrophil elastase (HNE), which is found in high concentration in chronic wound fluid. N-succinyl-alanine-alanine-proline-valine-p-nitroanilide (suc-Ala-Ala-Pro-Val-pNA), a chromogenic HNE substrate, was attached to glyci...

Although strategies of compositing noble materials and Zeolitic imidazolate frameworks (ZIFs) have been used to enhance the performance ZIF-based surface-enhanced Raman scattering (SERS) substrates, enhancement process still remains unclear sampling with powder-form substrates is challenging for practical applications. In this study, a flexible SERS substrate silver (Ag) nanoparticle decorated ...

Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to s...

in order to evaluate the effect of substrate and enrichment material on biological efficiency (be) and other physical and chemical propertiese of medicinal mushroom shiitake (lentinula edodes) an investigation was perfomed as factorial experiment in completely randomized designe with four replications in khorramabad city of lorestan continent iran. experimental factors were substrate in three l...

Carotenoids are fat-soluble bioactive compounds found in plants, animals, and microorganisms, which have a range of health benefits great technological industrial importance the fields pharmaceuticals, food, biotechnology. Yeasts genus Rhodotorula can biosynthesize large amounts carotenoids under environmental stress conditions, this process may become industrially feasible if production costs ...