Despite the tremendous advancement in somatic cell nuclear transfer (SCNT) camels, generation of cloned calf using SCNT cows is yet to be reported United Arab Emirates (UAE). The present study intended produce calves local environment UAE. We surgically collected ear tissue from two high-performance Holstein (average production 71 liter/ day) and established fibroblast line perform SCNT. Fifty-...

Mammalian oocytes can reprogram somatic cells into a totipotent state enabling animal cloning through somatic cell nuclear transfer (SCNT). However, the majority of SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we identify histone H3 lysine 9 trimethylation (H3K9me3) of donor cell genome as a major barrier for efficient reprogramming by SCNT. Comparative tra...

Nuclear, microtubular dynamics and spindle assembly checkpoint (SAC) in bovine somatic cell nuclear transfer (SCNT) oocytes receiving G1/0 or M phase somatic cell nuclei were studied. SCNT oocytes assembled microtubules, however, the spindles were structurally abnormal, including bi-, tri-polar or elongated spindles with scattered premature chromosome condensation (PCC) in G1/0 phase nuclei, an...

To transfer only part of genome, X-ray irradiated suspension cell protoplasts of N. Ian gsdorfii were fused with suspension cell protoplasts of N. glauca by polyethylene glycol. Somatic hybrid calli were selected by the growth in the hormone-free medium. Some of somatic hybrid calli from fusion with irradiated protoplasts indicated the loss of small subunit polypeptide of fraction 1 protein whi...

This review focuses on the platform of recent advances in reproductive biotechnologies in sheep and goat. Here, we discuss some aspects of veterinary and biomedical importance in artificial insemination using sexed semen, in vitro/in vivo fertilization, interspecies in vitro fertilization, multiple ovulation and embryo transfer (MOET), somatic cell nuclear transfer and transgenic animal product...