To clarify the interplay of kinetic hole-burning (KHB), structural relaxation, and ligand migration in myoglobin (Mb), we measured time-resolved absorption spectra in the Soret region after photolysis of carbon monoxide Mb (MbCO) in the temperature interval 120-260 K and in the time window 350 ns to 200 ms. The spectral contributions of both photolyzed (Mb*) and liganded Mb (MbCO) have been ana...

A study on heat and mass transfer behaviour on an anisotropic porous medium embedded in square cavity/annulus is conducted using Incompressibe Smoothed Particle Hydrodynamics (ISPH) method. In the case of square cavity, the left wall has hot temperature Th and mass Ch and the right wall has cool temperature Tc and mass Cc and both of the top and bottom walls are adiabatic. While in the case of ...

When rhodopsin in a gelatin film is dried, the rhodopsin chromophores orient primarily in the plane of the film. When the film is wetted, the chromophores disorient. These changes are reversible. When rhodopsin in a wet film. is bleached in the presence of hydroxylamine and redried, the retinal oxime which results is oriented more perpendicularly to the plane of the film. These orientations in ...

Thermal gradients induce concentration gradients in alkali halide solutions, and the salt migrates towards hot or cold regions depending on the average temperature of the solution. This effect has been interpreted using the heat of transport, which provides a route to rationalize thermophoretic phenomena. Early theories provide estimates of the heat of transport at infinite dilution. These valu...

Corroles are a developing class of tetrapyrrole-based molecules with significant chemical potential and relatively unexplored photophysical properties. We combined femtosecond broadband fluorescence up-conversion and fs broadband Vis-pump Vis-probe spectroscopy to comprehensively characterize the photoreaction of 5,10,15-tris-pentafluorophenyl-corrolato-antimony(V)-trans-difluoride (Sb-tpfc-F₂)...

Met80 of cytochrome c (cyt c) has been shown to dissociate from its heme iron when cyt c interacts with cardiolipin (CL), which triggers the release of cyt c into the cytosol initiating apoptosis. We found that the mass of human cyt c increases by 16 Da in the Met80-Lys86 region by reaction with molecular oxygen in the presence of CL-containing liposomes and dithiothreitol (DTT). To investigate...

GTN (nitroglycerin; glycerol trinitrate) causes dilation of blood vessels via activation of nitric oxide (NO)-sensitive sGC (soluble guanylate cyclase), a heterodimeric haem protein that catalyses the conversion of GTP into cGMP. Activation of sGC by GTN requires enzymatic or non-enzymatic bioactivation of the nitrate. Based on insufficient NO release and lack of spectroscopic evidence for form...

Thermal convection in colloidal suspensions of nanosized particles is investigated. Representative examples for such materials are ferrofluids, but since we do not imply any external magnetic field, the description applies to nonmagnetic suspensions as well. With the grain size being large on molecular length scales, the particle mobility is extremely small, allowing to disregard the concentrat...