Samples of gasoline engine oil (SAE 5W20) that had been exposed to various oxidation times were inspected with a UV-Visible (UV-Vis) spectrophotometer select the best wavelengths and wavelength ranges for distinguishing times. Engine samples subjected different thermal periods 0, 24, 48, 72, 96, 120, 144 hours, resulting in range total base number (TBN) levels. Each (190.5 – 849.5 nm) selected ...

Flower shaped antimony oxide (Sb2O3) microstructures were synthesized in a large quantity via simple solution method using aqueous mixtures of antimony chloride and hexamethylene diamine (HMDA). The morphological characterizations were done by field emission scanning electron microscopy (FESEM), which revealed that the synthesized products possess flower-shaped microstructures. The detailed str...

Fat-soluble vitamin regulation is of particular significance in cystic fibrosis. A vitamin is an organic compound required as a nutrient in tiny amounts by an organism. In the present study, we had developed a simple method to estimate the fat soluble vitamin by Spectrophotometer. The vitamin A, D, E and K were estimated by spectrophotometer at 325 nm, 264 nm, 525 nm and 635 nm respectively. Af...

A versatile, low-cost, digital signal processor (DSP) based lock-in module with external reference is described. This module is used to implement an industrial spectrophotometer for measuring spectral transmission and reflection of automotive and architectonic coating glasses over the ultraviolet, visible and near-infrared wavelength range. The light beams are modulated with an optical chopper....

I received my basic training in enzymology as a graduate student in the laboratory of Professor T. R. Hogness at the University of Chicago from 1936 to 1939. Hogness had constructed a photoelectric spectrophotometer modeled after the one in Otto Warburg’s laboratory in Berlin-Dahlem. I was assigned a problem on succinic dehydrogenase from beef heart, using the Warburg manometric apparatus, and ...