Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-...

BACKGROUND Genetic diversity of 19 forage-type and 2 turf-type cultivars of tall fescue (Festuca arundinacea Schreb.) was revealed using SSR markers in an attempt to explore the genetic relationships among them, and examine potential use of SSR markers to identify cultivars by bulked samples. RESULTS A total of 227 clear band was scored with 14 SSR primers and out of which 201 (88.6 %) were f...

The objective of the present study was to make use of efficient molecular marker systems to reveal genetic relationships in traditional and evolved Basmati (EB) and semidwarf non-Basmati (NB) rice varieties. A subset of three rice groups was analyzed by using 19 simple sequence repeat (SSR) loci and 12 inter-SSR-PCR primers. A total of 70 SSR alleles and 481 inter-SSR-PCR markers were revealed ...

Humulus lupulus is commonly known as hops, a member of the family moraceae. Currently many projects are underway leading to the accumulation of voluminous genomic and expressed sequence tag sequences in public databases. The genetically characterized domains in these databases are limited due to non-availability of reliable molecular markers. The large data of EST sequences are available in hop...

Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequ...

Article history: Received on: 26/11/2013 Revised on: 08/12/2013 Accepted on: 14/12/2013 Available online: 31/12/2013 Hexaploid triticale (x Triticosecale Wittmack) is a man-made hybrid that consists of 3 genomes, A, B and R, the Aand B-genome from wheat and the R-genome from rye. Simple sequence repeat (SSR) markers are transferable between closely related species. The aim of this study was to ...

SSR (Simple Sequence Repeat) markers and SRAP (Sequence Related Amplified Polymorphism) markers were used to construct a genetic linkage map of Vitis amurensis Rupr. The mapping population consists of 94 individuals derived from the selfing of Beibinghong, a V. amurensis cultivar. The map covers a total length of 1123cM on 21 linkage groups with an average distance between adjacent markers of 4...

In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking and sex-limited marking, respectively. ...

With the aim to increase the number of functional markers in resource poor crop like cultivated peanut (Arachis hypogaea), large numbers of available expressed sequence tags (ESTs) in the public databases, were employed for the development of novel EST derived simple sequence repeat (SSR) markers. From 16424 unigenes, 2784 (16.95%) SSRs containing unigenes having 3373 SSR motifs were identified...

Maize landraces represent potential sources of favorable traits for current and future breeding programs.. A comparative characterization of twenty-one flint maize landraces from Maize Research Institute "Zemun Polje“ gene bank was achieved by using morphological traits and SSR markers. Applied marker systems revealed high level of genetic heterogenity beetween landraces. Cluster analysis of th...