A crossbred calf (3 months old) obtained from a farm where regular control of ticks was practised and found to be free of blood parasites was inoculated with 20 ml pooled blood collected from four field cattle which had very low Trypanosoma theileri parasitaemias (one parasite per 70 microl blood as determined by the haematocrit centrifugation technique). Trypanosoma theileri was present in the...

INTRODUCTION The ML Flow test is an immunochromatographic assay that detects IgM antibodies against M. leprae-specific anti-phenolic glycolipid I (PGL-I). In addition to slit skin smears stained by the Ziehl-Neelsen technique, it can be helpful in the operational classification of leprosy patients for treatment purposes. OBJECTIVE This work studied the relationship between antibody levels as ...

OBJECTIVE Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. METHODS A total of 112 fecal specimens were examined by conventional methods: Direct...

OBJECTIVE To evaluate the diagnostic accuracy of the Xpert MTB/RIF assay for the detection of M. tuberculosis in pulmonary and extrapulmonary specimens and to compare it with conventional techniques. METHODS During a period of 10 months from December 2012 through September 2013, two hundred and forty five clinically TB suspects were enrolled for Xpert MTB\RIF assay. The cohort comprised of 20...

BACKGROUND Fine needle aspiration (FNA) cytology is a well-established diagnostic method based on the microscopic interpretation of often scant cytological material; therefore, experience, good technique and smear quality are equally important in obtaining satisfactory results. AIMS OF STUDY We studied the use of fresh surgical pathology specimens for making so-called mock-FNA smears with the...

Histidine-rich protein II (HRP2)-based malaria rapid diagnostic tests (RDTs) have shown high sensitivity and specificity for detecting Plasmodium falciparum malaria in a variety of study settings. However, RDTs are susceptible to heat and humidity and variation in individual performance, which may affect their use in field settings. We evaluated sensitivity and specificity of RDTs during routin...

The confirming diagnosis for tuberculosis as recommended by World Health Organization is by detecting the Acid Fast Bacilli bacteriologically in the respiratory specimens. In certain cases, where there is a strong clinical and radiological suspicion for tuberculosis and with repeated sputum samples showing negativity for AFB, bronchoscopic study with bronchial washings for the detection of AFB ...

Listeriosis is a sporadic disease of ruminants that causes meningoencephalitis, septicemia and abortion. In this study, the usefulness of immunoperoxidase technique was investigated in early diagnosis of cattle listerial abortions. For this purpose, 96 smears prepared from vaginal swab samples that were collected from aborting cattle were stained for L. monocytogenes by immunoperoxidase techniq...

OBJECTIVE: A rare case of LDD confirmed by biopsy is being reported. Review of the published cases shows that the disease usually manifests by the signs of increased intracranial pressure with inconstant cerebellar symptoms. MRI is the imaging modality of choice by now. As such, clinical problems after gross total or complete removal have not been reported so far, but herein we stress the impor...