A polymerase chain reaction (PCR) assay was developed and compared with standard methods for rapid detection of Burkholderia cepacia, a major industrial contaminant, in cosmetic and pharmaceutical raw materials and finished products. Artificially contaminated samples were incubated for 24 h in trypticase soy broth containing 4% Tween 20 and 0.5% soy lecithin. DNA was extracted from each sample ...

on the use of formamide and reduced incubation temperatures. Here we describe a modification of FoLT PCR which allows direct amplification from solid tissue samples without the need for any DNA purification steps. Two oligonucleotide primer combinations directed against the murine I82 microglobulin gene were used in this study. Using a standard PCR protocol it was not possible to amplify the ap...

BACKGROUND Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years. OBJECTIVE To assess the value of multiplex ...

BackgroundWe investigated the impact of baseline tumor infiltrating lymphocytes (TIL) density and TIL composition along with clinicopathological factors on pathologic complete response (pCR) rate in patients treated standard neoadjuvant chemotherapy ± targeted therapy for early breast cancer.MethodsA prospective non-interventional study luminal B, HER2+ triple- negative cancer was performed. In...

The International Financial Reporting Standard (IFRS) 9 relates to the recognition of an entity’s financial asset/liability in its statement, and includes expected credit loss (ECL) framework for recognising impairment. quantification ECL is often broken down into three components, namely, probability default (PD), given (LGD), exposure at (EAD). IFRS standard requires that model accommodates i...

Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...

Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from ma...

Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...