Alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide E. C. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. This enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. Isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...

BACKGROUND Recent studies suggest that resistant starch (effective in producing butyrate and lowering possibly toxic ammonia) is rapidly fermented in the proximal colon; the distal colon especially would, however, benefit from these properties of resistant starch. AIMS To determine whether wheat bran (a rich source of insoluble non-starch polysaccharides), known to hasten gastrointestinal tra...

Studies in this and other laboratories have disclosed a variety of starch-digesting rumen organisms, including Streptococcu bouis (Hungate et al., 1952), several cellulolytic strains (Hungate, 1950), and various non-cellulolytic bacteria (Gall et al., 1947; Gall and Huhtanen, 1951; Huhtanen and Gall, 1953a, b; Bryant and Burkey, 1953a,b; Jayko, 1953; McPherson, 1953; van der Wath, 1948) as well...

the sequencing batch reactors were developed to treat the actual wastewater from ethyleneglycol/ethylene oxide producing industry. four identical reactors with total and effective liquid volumes of 9and 7 l were operated respectively in parallel. laboratory experiments were conducted with different organicloadings of 500, 1000, 1500 and 3000 g-cod/m3.day and the performance of the reactors was ...

This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 109 CFU/g was achieved by signal factor and orthogonal experiments. After fermentation, the starch content in the yeast culture reduced from 32.2% ± 0.5% to 7.5% ± 0.2%, and the contents of crude protein and pe...

Studies in this and other laboratories have disclosed a variety of starch-digesting rumen organisms, including Streptococcu bouis (Hungate et al., 1952), several cellulolytic strains (Hungate, 1950), and various non-cellulolytic bacteria (Gall et al., 1947; Gall and Huhtanen, 1951; Huhtanen and Gall, 1953a, b; Bryant and Burkey, 1953a,b; Jayko, 1953; McPherson, 1953; van der Wath, 1948) as well...

A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 kDa. The optimum temperatu...

α-Amylase is one of the important enzymes in the starch-processing industry. However, starch processing requires high temperature, thus resulting in high cost. The high adsorptivity of α-amylase to the substrate allows this enzyme to digest the starch at a lower temperature. α-Amylase from Saccharomycopsis fibuligera R64 (Sfamy R64), a locally sourced enzyme from Indonesia, has a high amylolyti...

The effect of different carbon source on the efficiency of enhanced biological phosphorus removal (EBPR) from synthetic wastewater with acetate and two ratios of acetate/starch as a carbon source was investigated. Three pressurized pure oxygen sequencing batch reactor (POSBR) experiments were operated. The reactors (POSBR1, POSBR2 and POSBR3) were developed and studied at different carbon sourc...

Starch contains phosphate covalently bound to the C6-position (70 to 80% of total bound phosphate) and the C3-position (20 to 30%) of the glucosyl residues of the amylopectin fraction. In plants, the transient phosphorylation of starch renders the granule surface more accessible to glucan hydrolyzing enzymes and is required for proper starch degradation. Phosphate also confers desired propertie...