This paper investigates a possible practical solution for reasonable oxygen supply during the pluripotent stem cells expansion processes, where the stem cells propagate as aggregates in stirred-suspension bioreactors. Low glucose and low oxygen concentrations are preferred for efficient proliferation of pluripotent stem cells. However, strong oxygen limitation, especially inside of cell aggrega...

In this work we propose a simultaneous scheduling and control optimization formulation to address both optimal steady-state production and dynamic product transitions in multiproduct parallel continuous stirred tank reactors. The simultaneous scheduling and control problem for multiproduct parallel continuous reactors is cast as a Mixed-Integer Dynamic Optimization (MIDO) problem. The reactor d...

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was 1.9...

The present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from Tagetes erecta using lipases from the yeast of Yarrowia lipolytica, with the aim of obtaining free lutein. The optimal concentrations of seven nutrients, considering the production of lipases relative to biomass (Yp/x) as the response variable, were determined in flask fermentations. In...

The inherent non-linearity of citric acid fermentation from Aspergillus niger renders its control difficult, so there is a need to fine-tune the bioreactor performance for maximum production of citric acid in batch culture. For this, fuzzy logic is becoming a popular tool to handle non-linearity of a batch process. The present manuscript deals with fuzzy logic control of citric acid accretion b...

Numerous observations indicate a high flexibility of microbial communities in different biogas reactors during anaerobic digestion. Here, we describe the functional redundancy and structural changes of involved microbial communities in four lab-scale continuously stirred tank reactors (CSTRs, 39°C, 12 L volume) supplied with different mixtures of maize silage (MS) and sugar beet silage (SBS) ov...

Designing large-scale stirred bioreactors with performance closely matching the one achieved in lab-scale fermenters presents continuous challenge. In this contribution, dynamic modelling of the aerobic biocatalytic conversion process in viscous batch stirred tank reactor is developed. Its operation is illustrated by simulation of the interaction of fluid flow, mass transfer and reaction releva...

Extensive expansion of mesenchymal stem cells (MSCs) for cell-based therapies remains challenging since long-term cultivation and excessive passaging in two-dimensional conditions result in a loss of essential stem cell properties. Indeed, low survival rate of cells, alteration of surface marker profiles, and reduced differentiation capacity are observed after in vitro expansion and reduce ther...