The yields of unrepairable single-and double-strand breaks in the DNA of x-irradiated Chinese hamster cells were measured by low-speed neutral and alkaline sucrose density gradient sedimentation in order to investigate the relation between these lesions and reproductive death. After maximal single-strand remoining, at all doses, the number of residual single-strand breaks was twice the number o...

Due to their unique structural and mechanical properties, randomly-crosslinked polymer networks play an important role in many different fields, ranging from cellular biology industrial processes. In order elucidate how these properties are controlled by the physical details of network (\textit{e.g.} chain-length end-to-end distributions), we generate disordered phantom with crosslinker concent...

Melanomas are highly radioresistant tumors, mainly due to efficient DNA double-strand break (DSB) repair. Dbait (which stands for DNA strand break bait) molecules mimic DSBs and trap DNA repair proteins, thereby inhibiting repair of DNA damage induced by radiation therapy (RT). First, the cytotoxic efficacy of Dbait in combination with RT was evaluated in vitro in SK28 and 501mel human melanoma...

The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...

Mercury is a threatening pollutant in food, herein, we developed Tb3+-nucleic acid probe-based label-free assay for mix-and-read, rapid detection of mercury pollution. The utilized the feature light-up fluorescence terbium ions (Tb3+) via binding with single-strand DNA. ion, Hg2+ induced thymine (T)-rich DNA strand to form double-strand structure (T-Hg2+</s...

background: breast cancer is one of the most prevalent malignancies among women in various countries. her2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. her2 gene encodes an 185kda transmembrane glycoprotein with 1255 amino acids. this active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. these...

Objective: In this paper, we have introduced topoisomerase inhibitors, mechanism of action and types of them. DNA topoisomerases are ubiquitous enzymes that catalyze essential enzymes to solve the topological problems accompanying key nuclear processes such as DNA replication, transcription, repair and chromatin assembly by introducing temporary single or double strand breaks in the DNA. Result...

Objective: In this paper, we have introduced topoisomerase inhibitors, mechanism of action and types of them. DNA topoisomerases are ubiquitous enzymes that catalyze essential enzymes to solve the topological problems accompanying key nuclear processes such as DNA replication, transcription, repair and chromatin assembly by introducing temporary single or double strand breaks in the DNA. Result...

The emergence of the novel coronavirus, SARS-CoV-2, has highlighted need for rapid, accurate, and point-of-care diagnostic testing. Lab-on-a-Chip (LoC) devices offer possibility to run such tests at a low cost, while same time permitting multiplexed detection several viruses when coupled with microarray amplified products. Herein, we report development protocol qualitative through design approp...