To explore the relationships among Ia antigen expression, epidermal Langerhans cells, and the immunogenicity of skin allografts, cellophane tape-stripping was used in H-2 congenic and recombinant mice of defined immunogenetic disparity. Tape-stripping of murine abdominal wall skin achieved almost complete depletion of epidermal Langerhans cells within a few hours of application, as measured by ...

We report the use of non-invasive tape stripping to sample psoriatic lesional and non-lesional skin in 96 patients. The procedure was well tolerated with any discomfort described as mild; we did not observe any cases of Koebner phenomena at any non-lesional tape-stripped sites. Tape-harvested epidermis was extracted for RNA, which was profiled by semiquantitative reverse transcriptase-PCR. This...

BACKGROUND/PURPOSE To develop a modification of the stripping method allowing quick automated processing of corneocyte samples to study human skin conditions. Although the previous scrubbing technique and stripping method can provide sufficient information about human corneocytes, they are either subject to artifacts or involve time-consuming data processing. METHODS AND MATERIALS In the firs...

Skull-stripping refers to the separation of brain tissue from non-brain tissue, such as the scalp, skull, and dura. In large-scale studies involving a significant number of subjects, a fully automatic method is highly desirable, since manual skull-stripping requires tremendous human effort and can be inconsistent even after sufficient training. We propose in this paper a robust and effective me...

An easy, but effective, method has been proposed to detect and quantify the Pb(II) in the presence of Cd(II) based on a Bi/glassy carbon electrode (Bi/GCE) with the combination of a back propagation artificial neural network (BP-ANN) and square wave anodic stripping voltammetry (SWASV) without further electrode modification. The effects of Cd(II) in different concentrations on stripping respons...

Stress-response transcription factors such as NFκB turn on hundreds of genes and must have a mechanism for rapid cessation of transcriptional activation. We recently showed that the inhibitor of NFκB signaling, IκBα, dramatically accelerates the dissociation of NFκB from transcription sites, a process we have called "stripping." To test the role of the IκBα C-terminal PEST (rich in proline, glu...

An electroanalytical method has been introduced for highly sensitive determination of hydralazine hydrochloride (Hy-HCl) based on its oxidation at a glassy carbon electrode modified with multiwalled carbon nanotubes (MWCNT/GCE). Studies showed that the electrochemical oxidation of Hy-HCl was accompanied by adsorption and highly sensitive responses could be achieved by adsorptive stripping volta...

BACKGROUND The recovery of RNA from the upper epidermis by tape stripping yields variable RNA mass but has not been evaluated for its dependence on anatomical location. Gene expression at different body locations and the origin of RNA recovered by tape stripping have not been investigated. OBJECTIVES To characterize the recovery of RNA from different anatomical locations by tape stripping; to...

More than 500 million ocean freight container units are shipped annually between countries and continents. Residual levels of fumigants, as well as naturally occurring off-gassing chemicals emanating from the goods, constitute safety risks, which may affect uniformed workers upon entering the container. The aim of this study was to assess workers' exposure during stripping of containers and is ...

Accurate and consistent skull stripping of serial brain MR images is of great importance in longitudinal studies that aim to detect subtle brain morphological changes. To avoid inconsistency and the potential bias introduced by independently performing skull-stripping for each time-point image, we propose an effective method that is capable of skull-stripping serial brain MR images simultaneous...