This study aimed at characterizing changes in rumen bacteria abundance and fermentation profiles by artificial saliva (AS) pH, evaluating the potential modulatory role of Aspergillus oryzae culture (AOC) a simulation technique (RUSITEC) system. The treatment included high AS pH (pH 6.8) or low 5.5) according to McDougall’s method, was sustained changing composition (NaHCO3 from 9.8 1.96 g/L, Na...

A routine monitoring for subacute ruminal acidosis (SARA) on the individual level could support minimization of economic losses and ensuring animal welfare in dairy cows. The objectives this study were (1) to develop a SARA risk score (SRS) by combining information from different data acquisition systems generate an integrative indicator trait, (2) investigation associations SRS with feed analy...

Two trials were conducted to evaluate the influence of malic acid supplementation on ruminal fermentation. In Trial 1, six Holstein steers (300 kg) with ruminal cannulas were used in a crossover design experiment to study the influence of malic acid (MA) on ruminal metabolism during glucose-induced lactic acidosis. Treatments consisted of a 77% steam-flaked barley-based finishing diet supplemen...

The objectives were to determine the optimum inclusion of NaOH necessary to buffer the acidity of dried distillers' grains with solubles (DDGS) and its effects on digestibility, ruminal metabolism, and metabolic acidosis in feedlot steers. Rumen cannulated Angus-crossed steers were blocked by BW (small: 555 ± 42 kg initial BW, = 4; large: 703 ± 85 kg initial BW, = 4) over four 21-d periods in a...

Subacute ruminal acidosis (SARA) continues to be a common and costly metabolic disorder in high-producing dairy cows worldwide. The objective of this study was evaluate if increasing the concentration physically effective neutral detergent fiber (peNDF) diets can reduce risk SARA fed high-concentrate diet. Thirty second-parity Holstein mid lactation (131 ± 8.3 d milk) were randomly allocated 3 ...

INTRODUCTION When feeding grains to feedlot cattle, the goal is to maximize starch digestion while managing acidosis in order to provide optimal economic utilization of the finishing diet. Starch, the main component of grains, is digested primarily in the rumen with some digestion occurring in the small intestine and cecum. The small intestine is the preferred location of starch digestion to oc...

Dairy heifers were subjected to a non-life-threatening challenge designed to induce ruminal acidosis by feeding grain and sugar. Large among animal variation in clinical signs of acidosis, rumen metabolite concentrations, and the rumen microbiome occurred. This exploratory study investigates sources of the variation by examining associations between the genome, metabolome, and microbiome, albei...