Identification of metabolic attributes of drought resistance in cowpea using metabolite profiling and osmotic adjustment analysis H. Trindade, E. Rosa, P. Goufo* Universidade de Trás-os-Montes e Alto Douro, Portugal Plants tolerate drought by producing organic solutes, which act as compatible osmolytes that accumulate in the cytosol for osmotic adjustment, or osmoprotectants that stabilize cell...

Drought stress is a major environmental stress that restricts plant growth and production in the majority of agricultural fields of the world. The application of different fertilizers, especially biofertilizers and organic fertilizers, might play an important role in the production of medicinal plants in order to improve their resistance to deficit water stress. In order to evaluate the effects...

ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media....

We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance...

The bioprotective nature of disaccharides is hypothesized to derive from the modification of the hydrogen bonding network of water which protects biomolecules through lowered water activity at the protein interface. Using ultrafast fluorescence spectroscopy, we measured the relaxation of bulk water dynamics around the induced dipole moment of two fluorescent probes (Lucifer Yellow Ethylenediami...

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The effect of crystal size on VHP sugar colour was evaluated on a factory scale over the 1988/89 season at Sezela. Generally, smaller crystals produced lower sugar colour but the difference wasnot significant (at the 5% level) in the size range tested. Smallercrystals producedloweraffinated sugarcolourand the difference was significant at the 5% level. The mass of sugar in a full rail truck was...