α,β-Unsaturated amidines can be synthesized in high yield by rhodium(II) carboxylate catalyzed 1,2-H shift following denitrogenation of α-diazo amidines. Using a large ligand and an electron-poor N-sulfonyl group results exclusive Z-selectivity excellent suppressing side reactions involving intramolecular oxygen transfer.

Exposure to azide compounds causes inhibition of cytochrome c oxidase in the mitochondria, leading acute lethal poisoning. Although urgent pharmaceutical intervention is required for rescue from poisoning, no antidote exists worldwide. We hypothesized that methemoglobin (metHb) can be a promising material as an poisoning because metHb strongly bind azide. However, administration not feasible ow...

Herein we demonstrate the sensitive nature of human blood-brain barrier (BBB) endothelial cells to sodium azide and its gaseous product. Sodium azide is known to be acutely cytotoxic at low millimolar concentrations, hence its use as a biological preservative (e.g., in antibodies). Loss of barrier integrity was noticed in experiments using Electric Cell-substrate Impedance Sensing (ECIS) biosen...

Cysteine dioxygenase (CDO) is a mononuclear, non-heme iron-dependent enzyme that converts exogenous cysteine (Cys) to cysteine sulfinic acid using molecular oxygen. Although the complete catalytic mechanism is not yet known, several recent reports presented evidence for an Fe(III)-superoxo reaction intermediate. In this work, we have utilized spectroscopic and computational methods to investiga...

Covalent tethering of P(Ph)2N(C6H4C≡CH)2 ligands (P(Ph)2N(C6H4C≡CH)2 = 1,5-di-(4-ethynylphenyl)-3,7-diphenyl-1,5-diaza-3,7-diphosphacyclooctane) to planar, azide-terminated glassy carbon electrode surfaces has been accomplished using a Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) coupling reaction, using a BH3←P protection-deprotection strategy. Deprotected, surface-confined ligands were ...

A 47-year-old laboratory assistant ingested approximately 9 g of sodium azide powder and died 4 h later at a hospital. A high-performance liquid chromatographic method using diode-array detection has been developed for the determination of an azide benzoyl derivative in blood (after a simple deproteinization) and in several tissues (after homogenization in a neutral buffer and deproteinization ...

The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative phosphorylation, ATP-dependent, succinate-supported NAD+ reduction, and ATP-ind...

A variety of 1,3,2-oxazaborolidin-5-ones (OXB) derived from N-sulfonyl amino acids exhibit a high top-face selectivity in complexation with pyridines under thermodynamically controlled conditions.