The use of DNA probes for Shiga-like toxin I (SLT-I) and SLT-II for detection of SLT-producing Escherichia coli (SLTEC) in foods and calf fecal samples was evaluated. Enrichment cultures were prepared from food or fecal samples. Colonies formed by plating of enrichment cultures were probed for SLTEC by colony hybridization. Alternatively, enrichment cultures were analyzed for SLTEC presence by ...

Probably the most common approach in the industry, particularly for frozen blocks, is to drill and probe. This doesn’t really measure “freeze time”, but it does tell the operator what the approximate core temperature is at the end of the freezing cycle. “Temperature Measurement and Fish”, the Torry Advisory Note of J.Graham (1977), gives a good account of these practices. The procedure for dril...