At present, the study of the synovial fluid (SF) is a tool that is used frequently in specialized laboratories because it allows the establishment of diagnosis of crystal associated arthropathies, supports the diagnosis of septic arthritis and helps establish other rheumatologic diagnoses such as monoarthritis or joint effusion. The complete study of the SF includes the following analyses: 1. M...

A B S T R A C T A new type of enzyme hydrolyzing the elastase substrate siuccinyl-L-alanyl-L-alanyl-L-alanine-4-nitroanilide has been found in cell-free rheumatoid synovial fluid. Normal plasma and osteoarthritic synovial fluid contained relatively little enzyme. The pH optimum was 8.0. Unexpectedly, the enzyme activity was not due to leukocyte elastase or any proteinase bound to a2-macroglobul...

Introduction: Arthritis is the initial manifestation of many joint disorders. An analysis of synovial fluid has long been recommended as a routine procedure to assist in the diagnosis of arthritis. Objective: The present study was undertaken to correlate the clinical presentation and the results of synovial fluid analysis in arthritic conditions. Materials and Methods: In the present study, A t...

The diagnosis of synovial amyloidosis is based upon synovial biopsy. Synovial fluid (SF) in seven patients with amyloid arthropathy associated with chronic renal failure undergoing haemodialysis were studied. The SF and synovial samples of 10 consecutive patients with seronegative mono- or oligoarthritis served as controls. Six of the seven patients with amyloid positive synovial biopsy specime...

Macrophages are important mediators of chronic inflammation and are prominent in the synovial lining and sublining of patients with rheumatoid arthritis (RA). Recently, we demonstrated increased TLR2 and TLR4 expression and increased response to microbial TLR2 and TLR4 ligands in macrophages from the joints of RA. The current study characterized the expression of the 96-kDa heat shock glycoprot...

1. The cartilage-proteoglycan-degrading activity of synovial fluid cells from rheumatoid patients is primarily due to neutral protease activity; hyaluronidase does not contribute significantly. Evidence for these conclusions is adduced from electrophoresis of degradation products, from comparison of the proteoglycan-degrading and protease activities of extracts and from the effects of activator...

OBJECTIVES Epidemiological and experimental studies have established obesity to be an important risk factor for osteoarthritis (OA), however, the mechanisms underlying this link remains largely unknown. Here, we studied local inflammatory responses in metabolic-OA. METHODS Wistar rats were fed with control diet (CD) and high-carbohydrate, high-fat diet (HCHF) for period of 8 and 16 weeks. Aft...

7 Hall N D, Gillan A H. Effects of antirheumatic drugs on protein sulphydryl reactivity of human serum. J Pharm Pharmacol 1979; 31: 676-80. 8 Jellum E, Munthe E, Guldal G, Aaseth J. Fate of the gold and the thiomalate part after intramuscular administration of aurothiomalate to mice. Ann Rheum Dis 1980; 39: 155-8. 9 Munthe E, Guldal G, Jellum E. Increased intracellular glutathione during penici...

OBJECTIVE To assess the expression of Toll-like receptor 3 (TLR-3) protein in synovial tissues and cultured synovial fibroblasts obtained from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the consequences of stimulation of cultured synovial fibroblasts with TLR-3 ligands. METHODS TLR-3 expression in synovial tissues was determined by immunohistochemistry ...