The Sysmex NE-8000 is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabil...

A dependable automated method for the analysis of phospholipids would be highly desirable, because manual proceduresfortheir assay are long and tedious. One such procedure has been proposed by Whitley and Album foruse with an AutoAnalyzer (Technicon Instruments Corp., Tarrytown, N.Y. 10591). The chemical basis of this system is sound, and has been des#{243}ribed in detail (1). However, the meth...

A previously reported assay for serum gluCose, in which hexokinase and glucose-6-phosphate dehydrogenase were used, was improved to replace the light-sensitive phenazine methosulfate with “diaphorase,” providing a one-reagent system. The assay system was automated for use with standard AutoAnalyzer equipment and evaluated at 40 and 60 samples per hour with a 2:1 sample-to-wash ratio. Sensitivit...

We performed a study on 210 pregnant women to determine platelet reference ranges and to evaluate differences with a control group of 210 healthy non–pregnant women using the Sysmex SE-9500. During the second and third trimesters of pregnancy a decrease in platelet count (PLT) occurred but this was not statistically significant. However, during the same period statistically significant increase...

Serum sodium and potassium analyses by continuous-flow flame photometry is a well-accepted procedure (1, 2). Recently Habig and Williamson (3) have used the widely available Instrumentation Laboratory Model 143 (IL-143) flame photometer for continuousflow analyses. Pennacchia et al. (4) have operated this flame photometer at rates as high as 120 samples per hour. In both systems the results app...

A fully automated method has been developed for the determination of total cholesterol in blood serum, using the AutoAnalyzer system. According to the new method, based on the cholesterol determination of Abell et a!. (1), the serum sample is saponified and subsequently extracted with carbon tetrachloride. After treatment with Liebermann-Burchard reagent, the amount of cholesterol is determined...

Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with ...