In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

BACKGROUND The aim of our experimental work was to assess morphological changes of arterial wall that arise during different thawing protocols of a cryopreserved human aortic root allograft (CHARA) arterial wall. METHODS The experiment was performed on CHARAs. Two thawing protocols were tested: 1, CHARAs were thawed at a room temperature at +23°C; 2, CHARAs were placed directly into a water b...

BACKGROUND The clinical efficacy of the current methods used for cryopreservation of metaphase II human oocytes is low. Meiotic spindle disorders are thought to be largely responsible for this situation. METHODS Supernumerary fresh metaphase II human oocytes were cryopreserved in 1,2-propanediol with 0.1 M sucrose using a slow freezing/rapid thawing programme. Meiotic spindles were analysed i...

BACKGROUND Cryopreservation of embryos may lead to zona hardening that may compromise in vivo hatching and implantation following thawing and transfer. Assisted hatching (AH) has been advocated as a means of assisting the natural hatching process and enhancing implantation. METHODS The aim of this study was to assess in a prospective randomized manner the effect of laser-assisted hatching (LA...

Abstract. The basal thermal state (frozen or thawed) of the Greenland Ice Sheet is under-constrained due to few direct measurements, yet knowledge this becoming increasingly important interpret modern changes in ice flow. first synthesis relied on inferences from widespread airborne and satellite observations numerical models, for which most underlying datasets have since been updated. Further,...

BACKGROUND Polycystic ovary syndrome (PCOS) patients are at increased risk of pregnancy complications, which may impair pregnancy outcome. Transfer of fresh embryos after superovulation may lead to abnormal implantation and placentation and further increase risk for pregnancy loss and complications. Some preliminary data suggest that elective embryo cryopreservation followed by frozen-thawed em...

In this study, the physicochemical and sensory quality characteristics due to the influence of various thawing methods on electro-magnetic and air blast frozen pork were examined. The packaged pork samples, which were frozen by air blast freezing at -45℃ or electro-magnetic freezing at -55℃, were thawed using 4 different methods: refrigeration (4±1℃), room temperature (RT, 25℃), cold water (15℃...

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at differen...

BACKGROUND The reasons for increased birth defect prevalence following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are largely unknown. Classification of birth defects by pathology rather than organ system, and examination of the role of embryo freezing and thawing may provide clues to the mechanisms involved. This study aimed to investigate these two factors. MET...

The current study investigated the relationship between the level of expression and tyrosine phosphorylation of the sperm protein 32 (sp32) and the activation of the boar proacrosin/acrosin system. The acrosomal membrane proteins of boar sperm for use in different treatment experiments (i.e., fresh sperm, freezing and thawing, capacitation, and acrosome reaction) were separated, stained by Coom...