BACKGROUND Bovine theileriosis results from infection with obligate intracellular protozoa of the genus Theileria. The phylogenetic relationships between two isolates of Theileria annulata, and 36 Theileria spp., as well as 6 outgroup including Babesia spp. and coccidian protozoa were analyzed using the 18S rRNA gene sequence. METHODS The target DNA segment was amplified by PCR. The PCR produ...

The study was conducted to assess the prevalence of Theileria infection in Hyalomma tick vectors collected from cattle host to record the natural infection level of theilerial parasite in the field condition. A total of 174 male and 298 female of Hyalomma marginatum issaci ticks were collected from three districts Sunsari, Morang and Jhapa of Eastern Terai of Nepal during 2004-2005. These Hyalo...

Serious disease outbreaks in cattle caused by Theileria orientalis have emerged in the Asia-Pacific region. Genetic variables of the major piroplasm surface protein (MPSP) expressed on the surface of the piroplasm inside T. orientalis-infected erythrocytes are considered to be associated with variation in the pathogenicity of T. orientalis. Our study describes the clinically relevant MPSP types...

bovine tropical theileriosis caused by theileria annulata is an economically important disease of cattle. an enzyme linked immunosorbent assay (elisa) was used to determine antibody levels in vaccinated and unvaccinated cattle, us¬ing cellular schizont as antigen and its results were compared with immunofluo-rescent assay (ifa). for this test 126 sera collected (105 vaccinated, 31 not vacci¬nat...

Are more dangerous strains of tuberculosis spreading in Southern Africa? LSHTM investigators: Peter Godfrey-Faussett, Ruth McNerney, Kim Mallard, Helen Ayles. Collaborators: ZAMBIA: ZAMBART Project; University Teaching Hospital, Lusaka; MOH Chest Diseases Laboratory. Malawi: Community Health Sciences Unit of the Ministry of Health and National TB Programme; EQUI –TB Knowledge Programme (Liverpo...

A case of bovine cerebral theileriosis was confirmed at autopsy on a farm where 4 animals out of 70 died. All were less than 2 years old and all showed nervous signs. Serologically, no evidence was found of Theileria mutans or the Theileria parva group in young animals born on the farm. Six out of 13 calves 6-9 months of age were, however, serologically positive for Theileria? taurotragi and it...

Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specif...