Jamun fruit comprises of seed and thick pulp. The pectin-protein bond of the thick pulp creates difficulty in making juice. Clear Jamun juice is not available in the market, so there is a need for extraction of juice with maximum yield. The goal of this research is to obtain high yield of clarified juice with the help of Pectinase (Aspergillus aculeatus) enzyme. The study was conducted at diffe...

Several atoxigenic Aspergillus flavus isolates, including some being used as biocontrol agents, and one toxigenic isolate were surveyed for the ability to produce extracellular xylanolytic and pectinolytic hydrolases. All of the tested isolates displayed good production of endoxylanases when grown on a medium utilizing larch xylan as a sole carbon substrate. Four of the tested isolates produced...

An improved enzymatic cotton scouring process has been developed on the basis of a fast enzyme reaction to efficiently remove the pectin and wax compounds from the organic cotton fabrics. An attempt has been made to study the pectinolytic activity of degrading rate of pectin on the organic cotton fabric using alkaline pectinase enzyme with various process parameters such as enzyme concentration...

Here we describe a half-semester biochemistry laboratory course based on the expression, isolation, purification, and biochemical characterization of RecJ nuclease. The students transformed expression plasmid thermostable nuclease into host bacteria Escherichia coli BL21(DE3), to induce recombinant by IPTG. expressed was purified from cell lysates broken with sonication Ni-affinity chromatograp...

Robust vaccines are required to protect village chickens against Newcastle disease. Thermostable vaccines derived from avirulent Australian strains of Newcastle disease virus (strains V4 and I-2) have proved successful for this purpose. These vaccines have been developed through ACIAR projects and have been adopted by other international aid agencies. It is now possible to offer integrated work...

We report the isolation, cloning and expression, in Bacillus subtilis, of the gene coding for thermolysin, a thermostable metalloprotease which is produced by Bacillus thermoproteolyticus Rokko. The nucleotide sequence has revealed that, like neutral proteases produced by other members of the Bacillus species, thermolysin is probably produced as a preproenzyme carrying a typical N-terminal memb...