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The role of sulfur on the availability of Cu and the bacterial community in rice rhizospheres was investigated by pot experiments. With sulfur addition, pH in rhizosphere soil decreased and Mg(NO₃)₂ extractable Cu increased significantly. The bacterial community composition also changed with sulfur addition. Some specific clones having high similarity to Thiobacillus, which indicated that sulfu...

The growth of Thiobacillus ferrooxidans on sulphur is known to proceed through the attachment of cells to the sulphur particles. Experiments, However, show that the cells in the liquid phase, which are not attached to the sulphur particles, also grow. It has been shown through the use of a two-compartment membrane reactor that this increase is partially due to the release of ions, corresponding...

The oxidation of elemental sulfur by Thiobacillus thiooxidans was studied at pH 2.3, 4.5, and 7.0 in the presence of different concentrations of various anions (sulfate, phosphate, chloride, nitrate, and fluoride) and cations (potassium, sodium, lithium, rubidium, and cesium). The results agree with the expected response of this acidophilic bacterium to charge neutralization of colloids by ions...

The growth yield of Thiobacillus intermedius is greater in glucose-yeast extract or glucose-casein hydrolysate broth than in comparable media without glucose. The quantity of glucose utilized in the glucose-supplemented media is much greater than the increase in cell yield observed relative to the unsupplemented media. Addition of glucose to cell-free extracts of glucose-yeast extract or glucos...

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. S...

effect of different amounts of sulfur fertilizer (0, 50, 100, 150, 200, 250 kg/ha) and 200 kg/ha of thiobacillus inoculant on yield, quality and storability of two edible onion cultivars red rey and white qom were investigated. the experiments were conducted at experimental field and laboratories of the department of horticulture, seed and plant improvement institute, karaj, iran in 2007-08 cro...

Compacted bentonites are one of the best sealing and backfilling clays considered for use in Deep Geological Repositories radioactive wastes. However, an in-depth understanding their behavior after placement repository is required, including if activity indigenous microorganisms affects safety conditions. Here we provide optimized phenol:chloroform based protocol that facilitates higher DNA-yie...

The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10 to 10 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidan...

sesame is one of the oldest crops in the world that is commonly cultivated in arid and semi-arid due to high oil seeds (42-52 %). this study was conducted to investigate the effects of sulfur and biological fertilizers on physiological parameters of sesame at sari agriculture sciences and natural resources university in 2014 crop year. the experiment was conducted in a split factorial design ba...