1. Introduction To identify proteins, a de novo sequencing algorithm computes the peptide sequences from MS/MS data without the need of a protein database. When proteins are heavily modified or from an organism whose genome is not sequenced, de novo sequencing is the only reliable approach to identify the proteins in a sample. De novo sequencing typically requires higher quality data than those...

Analysis of the N-glycosylation pattern on antibodies is described using complementary mass spectrometric strategies based on both, top-down ESI-UHR-TOF and bottom-up LC-MALDI-TOF/TOF. Fast LC-ESI-UHR-TOF analysis, performed on the Bruker maXisTM, provides highresolution, high-mass accuracy (confident low ppm) data for both, intact antibodies and released antibody heavy chains allowing a rapid ...

The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three sp...

Two types of amorphous, silica nanoparticles have been produced and used as surface assisting agents during laser desorption/ionisation time-of flight-mass spectrometry (SALDI-TOF-MS). The first is hydrophilic possessing surface aminopropyl groups and the second hydrophobic containing surface phenyl groups. Each particle type acts as a solid phase adsorbent, adsorbing analytes according to thei...

Metabolic profiling of biological samples is increasingly used to obtain more insight into the pathophysiology of diseases. For translational studies, biological samples from animal models are explored; however, the volume of these samples can be a limiting factor for metabolic profiling studies. For instance, only a few microliters of urine is often available from small animals like mice. Henc...

In recent years, MALDI-TOF Mass Spectrometry (MS) method has emerged as a promising and a reliable tool for bacteria identification. In this study we compared Bruker MALDI-TOF MS and conventional phenotypic methods to identify a collection of 333 Gram-positive clinical isolates comprising 22 genera and 60 species. 16S rRNA sequencing was the reference molecular technique, and rpoB gene sequecin...