A modification of the serum glutamic-pyruvic transaminase colorimetric assay is described which reduces the error due to preformed keto acids, and thus permits evaluation of “corrected” transaminase levels in serum specimens that have stood on the clot up to three weeks at 40 Evidence is presented that even in bacterially contaminated specimens the major cause of elevation in levels during stor...

We have studied the effect of cold shock on the percentage of enzyme release in buffalo spermatozoa, i.e. alcohol, malic, glucose-6-phosphate, isocitric, lactate, sorbital dehydrogenases, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), hyaluronidase, arylsulphatase A and arylsulphatase B. Significant amounts of alcohol, malic and sorbital dehydrogenases, GOT, hyalu...

Minocycline (25 to 100 micrograms/g) dose dependently increased serum glutamic oxalacetic transaminase, urea, and bilirubin levels, and the hepatic triglyceride content in mice. In animals pretreated with phenobarbital to enhance minocycline metabolism, the effects on liver triglycerides were attenuated, while the changes in serum glutamic oxalacetic transaminase, urea, and bilirubin were enhan...

The hepatoprotective activity of a fraction of the leaf extract of A.indica against carbon tetrachloride : liquid paraffin (1:1) induced liver damage in rats at doses of 100 mg/kg and 200 mg/kg was evaluated. A significant dose dependent hepatoprotective activity was evidenced by lowering of the elevated levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT),...

TRANSAMINASES are among the enzyme requiring pyridoxal-5 phosphate as a co-factor (1). Tn the course of studies on the presence of the apoenzyme of glutamic oxaloacetic transaminase (GOT) in the serum, it was found that the malic dehydrogenase preparations, which are used in the Karmen (2) transaminase assay system, had variable amounts of apotransaminase. The studies reported below present the...

Vigabatrin and gabaculine, both highly specific inhibitors of GABA (gamma-aminobutyric acid) transaminase, cause significant elevation of endogenous GABA levels in brain. The time course of GABA concentration after acute GABA transaminase inhibition was measured quantitatively in the alpha-chloralose-anesthetized rat brain using in vivo selective homonuclear polarization transfer spectroscopy. ...

1. Experiments were designed to establish the relationship between renal gluconeogenesis and sodium transport. Six inhibitors of gluconeogenesis, with different sites of action, were tested in the isolated perfused rat kidney in which renal function, and in particular Na+ reabsorption, was followed. 2. In kidneys from fed or starved rats specific inhibitors of gluconeogenesis neither inhibited ...

Because of the general nature of the transamination reaction (1, 2), a study of the kinetics and mechanism of reaction seemed of interest. Preliminary studies of this nature have already appeared (3-5). However, these studies were carried out with crude extracts or resolved enzyme systems. In order to conduct a critical kinetic study as well as a study of the mechanism of reaction, large amount...

Purified D-amino acid transaminase from Bacillus sphaericus catalyzes an (~,/3 elimination from the D isomer of pchloroalanine to yield equivalent amounts of pyruvate, chloride, and ammonia; the L isomer of chloroalanine is not a substrate for this transaminase. During the /I elimination there is a synchronous loss in enzyme activity; the Kinact for /I-chloroalanine was estimated to be about 10...

Effects of nutritional and hormonal treatments on the activities of rat liver histidine-catabolizing enzymes histidine pyruvate transaminase, histidase, and urocanase, have been examined and estimates have been made of the rates of induction and degradation of each enzyme. Histidase and urocanase activities were slightly elevated in animals fasted for 48 hours, but the transaminase was not affe...