BACKGROUND The purpose of this study was to demonstrate the potential of magnetic poly(methyl methacrylate) (PMMA) core/polyethyleneimine (PEI) shell (mag-PEI) nanoparticles, which possess high saturation magnetization for gene delivery. By using mag-PEI nanoparticles as a gene carrier, this study focused on evaluation of transfection efficiency under magnetic induction. The potential role of t...

INTRODUCTION We have developed genetically engineered stable transfected cell lines, expressing hormone receptor and luciferase based reporter genes, for screening compounds and compound mixtures for endocrine disrupter activity. We have named the lines " ER-EcoScreen™ " (expressing estrogen receptor) and " AR-EcoScreen™ " (expressing androgen receptor). To establish these cell lines we introdu...

MicroRNA-204 (miR-204) has been reported to be frequently downregulated in various types of cancer, including renal, brain, ovary, hematological and colon cancer. The present study, investigated the effects of miR‑204 on renal cell carcinoma. Following transfection of miR‑204, an MTT assay, cell migration assay, cell invasion assay, western blot analysis and luciferase assay were performed in r...

In this report, we compare the function of the human beta-globin locus control region (LCR) in three K562 erythroleukemia cell assays, including (1) a transient transfection assay for "classical" enhancer activity, (2) a colony assay that detects "productive integration events," and (3) an assay that detects the ability of LCR fragments to confer hemin inducibility on linked, stably integrated ...

in this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (aat) with correct post-translational modifications, a protein production system based on the transduction of cho and cos-7 cells using lentiviral vectors was developed. human aat cdna was cloned into a replication-defective lentiviral vector. the transgene aat-jred chimer was transferr...

Abstract Background Growth factors execute essential biological functions and affect various physiological pathological processes, including peripheral nerve repair regeneration. Our previous sequencing data showed that the mRNA coding for betacellulin (Btc), an epidermal growth factor protein family member, was up-regulated in rat sciatic segment after injury, implying potential involvement of...

Background: Endometriosis is a painful disorder characterized by the growth of endometrial tissue outside uterine cavity. Here, we investigated effects cinnamic acid isolated from Chinese medicinal plant Cinnamomum cassia Presl on primary stromal cells. Methods: Immunohistochemistry was used to examine protein expression and cell purity. Quantitative RT-PCR conducted assess mRNA expression, Wes...

We aimed to investigate the effects of puerarin on high glucose (HG)-induced human retinal endothelial cells (HRECs) injury. Cells were exposed in presence or absence HG challenge. Cell viability was determined using a CCK-8 assay. Then, levels oxidative stress-related factors evaluated corresponding kits. Pyroptosis assessed by measurement gasdermin-N domain (GSDMD-N) and caspase-1 (CASP1) exp...

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its in CHO-K1 cells and the inhibition effect pig on PRRSV proliferation verified. The plasmid pEGFP-PoIFN-λ1 from gene fragment transfected into cells. Expression detected by fluorescence microscopy Western blotting. influence assessed. results study showed that recombinant correctly. After transfection, green f...

BACKGROUND Honokiol, a small-molecular weight natural product, has previously been reported to activate apoptosis and inhibit gastric tumorigenesis. Whether honokiol inhibits the angiogenesis and metastasis of gastric cancer cells remains unknown. METHODOLOGY/PRINCIPAL FINDINGS We tested the effects of honokiol on angiogenic activity and peritoneal dissemination using in vivo, ex vivo and in ...