Model Driven Engineering aims at improving effectiveness and efficiency of software engineering. Model transformations are a key artifact in model driven engineering as they enable transforming models into other artifacts for further processing, e.g. analysis models or executable code. However, particularly graph transformation based model transformation tools often lack detailed debugging capa...

Model transformations are important in Model Driven Engineering (MDE). They automate software development steps and greatly improve productivity and reduce software errors. However, the design of model transformation rules requires lots of manual work. To fully take advantage of MDE, correctness of model transformation rules should be ensured. In this paper, we present an ongoing work to use mo...

This protocol describes a simple and efficient procedure for preparation and storage of competent Escherichia coli cells applicable to transformation and transfection. This procedure is much simpler and more reproducible than other similar methods, and allows much higher transformation efficiency (1) even after 3 months storage of the competent cells. It is also superior to the electroporation ...

We report here the effect of antibiotics on the regeneration potential of recalcitrant indica rice cultivar, IR64. Different protocols reporting high-efficiency agro-bacterium-mediated transformation of mature seed-derived regenerative calli were used and compared. The putative transgenic (T0) plants were analyzed for integration of the transgene through polymerase chain reaction and Southern b...

In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA. Both glucose and rich nutrients present in the SOC medium prevented the unintended activation of the lac promoter carried on the ...

The relative contribution of DNA restriction and of sequence heterology as barriers to interspecies transfer of DNA was studied in the heterologous transformation of Bacillus subtilis recipients by DNA was studied in the heterologous transformation of Bacillus subtilis recipients by DNA isolated from B. globigii. Transformants were obtained at very low frequencies in the evolutionarily nonconse...