Jasmonic acid (JA) is an important plant hormone involved in regulation of many aspects of plant growth and development including secondary metabolism and JASMONATE ZIM-DOMAIN (JAZ) proteins are key components in JA signal processes. In this study, two new JAZ genes named SmJAZ3 and SmJAZ9 were cloned from S. miltiorrhiza hairy roots and characterized. Expression profiles under methyl jasmonate...

The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculati...

The family of chloride channel proteins that mediate Cl(-) transportation play vital roles in plant nutrient supply, cellular action potential and turgor pressure adjustment, stomatal movement, hormone signal recognition and transduction, Cl(-) homeostasis, and abiotic and biotic stress tolerance. The anionic toxicity, mainly caused by chloride ions (Cl(-)), on plants under salt stress remains ...

Background and objectives: Many Nepeta species have been commonly used in Iranian folk medicine as tranquilizer, relaxant, carminative and restorative tonic for nervous and respiratory disorders. Inflammation is a problem in many diseases and has an important role in brain function that can cause neurodegenerative disorders. Inflamed glial cells can exacerbate neurodegenerative diseases by prod...

Hairy root cultures of Mitragyna speciosa were established by infection of Agrobacterium rhizogenes ATCC 15834 and maintained in McCown woody plant medium (WPM) supplemented with 0.5 mg/1 naphthaleneacetic acid. The hairy roots were identified for the rooting genes loci of rolA and rolB by polymerase chain reaction. For studying the secondary metabolite production, the n-hexane extract of the h...

Similar to Agrobacterium tumerfaciens, Agrobacterium rhizogenes can transfer foreign DNAs into plant cells based on the autonomous root-inducing (Ri) plasmid. A. rhizogenes can cause hairy root formation on plant tissues and form composite plants after transformation. On these composite plants, some of the regenerated roots are transgenic, carrying the wild type T-DNA and the engineered binary ...

Rosmarinic acid (RA) is a multifunctional caffeic acid ester present in sweet basil (Ocimum basilicum L.). Untransformed normal roots of O. basilicum harbored the maximum titers (0.98% g fresh weight basis) of RA compared to leaves and shoots. Hairy root cultures of O. basilicum transformed with Agrobacterium rhizogenes (ATCC-15834) showed three-fold increases in growth and RA production compar...

Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a...

Malate accumulation has been suggested to balance Al-induced citrate synthesis and efflux in soybean roots. To test this hypothesis, characteristics of Al-induced accumulation and efflux of citrate and malate were compared between two soybean genotypes combining a functional analysis of GmME1 putatively encode a cytosolic NADP-malic enzyme. Similar amounts of citrate were released, and root elo...

Linum spp. (Linaceae) has been shown to accumulate cytotoxic lignans such as podophyllotoxin and 6-methoxypodopyllotoxin. Therefore, plant cell and tissue cultures of Linum spp. may be possible alternative sources for the lignan production. In this study, we focused on the production of lignans by root cultures of Linum spp. Hairy root cultures of L. nodiflorum, L.album and L. austriacum and ro...