An LC-MS/MS method was developed and validated to be used as a stability indicating assay for the study of a 3 mg/mL lansoprazole oral suspension. The method utilizes a UPLC (ultra-performance liquid chromatography) column and unique mass spectrometric detection (ion-trap time-of-flight (IT-TOF)) to achieve a sensitive (LOD 2 ng/mL), accurate, and reproducible quantification of lansoprazole. Th...

The universal mercury vapor black light trap is an effective device used for collecting moth specimens in a wide variety of habitats; yet, they can present challenges for researchers. The mercury vapor trap is often powered by a heavy automotive battery making it difficult to conduct extensive surveys in remote regions. The mercury vapor trap also carries a considerable financial cost per trap ...

Broomrapes are obligate root parasitic weeds and major constraint to many dicotyledoncrops especially in Mediterranean agricultural lands. Due to the fact that no single controlmeasure is able to control broomrapes in the field satisfactorily, integrated management isrecommended in general. The use of of trap crops or catch crops is an important strategy forcontrolling the weeds; and allelopath...

Objective: To evaluate telomerase activity as a marker of lymphocyte proliferation in systemic lupus erythematosus (SLE). Methods: CD19+, CD4+, and CD8+ lymphocytes were isolated from the peripheral blood of nine patients with SLE and nine healthy controls by means of magnetic beadcoupled antibodies and tested for telomerase activity with the TRAP assay. Results: Telomerase activity was signifi...

Purpose To identify the role of the fragment crystallizable (Fc) region in determining intraocular protein drug pharmacokinetics. Methods We generated a new VEGF-Trap lacking the Fc region (FcfVEGF-Trap, MWt = 100 kDa) by replacing the Fc region of native VEGF-Trap (MWt = 145 kDa) with a dimerized coiled-coil domain. Forty-two rabbits were injected intravitreally with VEGF-Trap or FcfVEGF-Tra...

Optical trapping experiments of different complexities are making a significant impact in biology. This review seeks to highlight design choices for scientists entering the field or building new instruments and to discuss making calibrated measurements with optical traps. For specificity, this review focuses on nucleic acid-based assays, but the discussion reflects the general experimental desi...

The traditional method for recording electrophysiological signals from the C. elegans pharynx is called an electropharyngeogram (EPG). It provides an experimental platform for defining gene function, the impact of genetic mutations, and the effects of drugs on the nematode neural network. To date, the EPG has been a low-throughput assay. This paper describes a new microfluidic device that can s...

Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detect...

A number of proteins form covalent bonds with DNA as obligatory transient intermediates in normal nuclear transactions. Drugs that trap these complexes have proven to be potent therapeutics in both cancer and infectious disease. Nonetheless, current assays for DNA-protein adducts are cumbersome, limiting both mechanistic studies and translational applications. We have developed a rapid and sens...