نتایج جستجو برای: trf
تعداد نتایج: 767 فیلتر نتایج به سال:
In human cells, transfection of telomeric T2AG3 repeats induces the formation of functional telomeres at previously interstitial sites. We report that telomere formation has stringent sequence requirements. While (T2AG3)n telomere seeds formed telomeres in approximately 70% of the transfected cells, five T2AG3-related heterologous telomeric DNAs seeded new telomeres in < 5% of the transfectants...
A novel light-emitting monomer M1 and its side-chain polymer P1 containing three conjugated aromatic pendants, including one pyridyl terminus, were successfully synthesized via Wittig and Pd-catalyzed Heck coupling reactions. The fluorescence of polymer P1 was efficiently quenched upon addition of different metal ions due to the facile energy transfers from the pendent groups of polymer P1 to s...
Telomere length measurement is an essential test for the diagnosis of telomeropathies, which are caused by excessive telomere erosion. Commonly used methods are terminal restriction fragment (TRF) analysis by Southern blot, fluorescence in situ hybridization coupled with flow cytometry (flow-FISH), and quantitative PCR (qPCR). Although these methods have been used in the clinic, they have not b...
Multidecadal stability in tropical rain forest structure and dynamics across an old-growth landscape
Have tropical rain forest landscapes changed directionally through recent decades? To answer this question requires tracking forest structure and dynamics through time and across within-forest environmental heterogeneity. While the impacts of major environmental gradients in soil nutrients, climate and topography on lowland tropical rain forest (TRF) structure and function have been extensively...
Telomerase activity was examined in 170 human brain tumor tissues, and terminal restriction fragment (TRF) length was examined in 152 of the 170. Telomerase activity was detected in 61.7% (66 of 107) of the neuroepithelial tumors. However, the detection rates of telomerase activity were widely different for different histopathological entities. In the case of astrocytic tumors, the detection ra...
FIELD INHOMOGENEITY COMPENSATION IN HIGH FIELD MAGNETIC RESONANCE IMAGING (MRI) Zhenghui Zhang, PhD University of Pittsburgh, 2006 This thesis concentrates on the reduction of field (both main field B0 and RF field B1) inhomogeneity in MRI, especially at high B0 field. B0 and B1 field inhomogeneity are major hindrances in high B0 field MRI applications. B1 inhomogeneity will lead to spatially v...
Acute phase proteins play a significant role in anemia of chronic disease and thought to be beneficial in maintain homeostasis. In both the condition, anemia of chronic disease as well as iron deficiency anemia alteration in iron homeostasis takes place. The study includes patients of pulmonary tuberculosis patients (ACD), Iron deficiency anemia (IDA) patients and control subjects. Serum CRP, S...
Leaf Area Index (leaf area per unit ground area, LAI) is a key driver of forest productivity but has never previously been measured directly at the landscape scale in tropical rain forest (TRF). We used a modular tower and stratified random sampling to harvest all foliage from forest floor to canopy top in 55 vertical transects (4.6 m(2)) across 500 ha of old growth in Costa Rica. Landscape LAI...
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